What is Hsqc Tocsy?

What is Hsqc Tocsy?

An HSQC-TOCSY experiment stands for Heteronuclear Single Quantum Coherence-Total Correlation Spectroscopy and other variants include HMQC-TOCSY, HSQC-COSY, etc. Depending on the mixing time, the hybrid experiment generally offers information on both short-range and long-range coupled nuclei.

How does HSQC work?

HSQC works by transferring magnetization from the I nucleus (usually the proton) to the S nucleus (usually the heteroatom) using the INEPT pulse sequence; this first step is done because the proton has a greater equilibrium magnetization and thus this step creates a stronger signal.

How does HSQC NMR work?

How long does an HSQC take?

The HSQC experiment is a highly sensitive 2D-NMR experiment and was first described in a 1H—15N system, but is also applicable to other nuclei such as 1H—13C and 1H—31P.

What is the difference between COSY and HSQC?

All Answers (7) Dear Hicham, COSY is a 2D spectrum that shows scalar coupling between vicinal H atoms (via 3 bonds). On both axis you have 1H spectrum and you can see the exact correlations and it can help you resolve structure. HSQC on the other hand shows correlation between C and H that is bonded to it (via 1 bond).