How do DNA methylation arrays work?
Methylation arrays enable quantitative interrogation of selected methylation sites across the genome, offering high-throughput capabilities that minimize the cost per sample. Array-based methylation studies can provide valuable insights into the regulation of gene expression.
How does methylation microarray work?
The Human DNA Methylation microarrays enable analysis of DNA methylation and a greater understanding of the epigenetic events that regulate gene expression and key biological processes. In addition, we provide a suite of microarray designed to analyze protein, with ChIP on Chip technologies. Read on to learn more.
What happened to Affymetrix?
Affymetrix is now Applied Biosystems, a brand of DNA microarray products sold by Thermo Fisher Scientific that originated with an American biotechnology research and development and manufacturing company of the same name.
What is an epic array?
The Infinium methylation EPIC array allows the interrogation of methylation patterns at the genome-wide level, covering more than 850,000 methylation sites. This array has shown to be very popular and is an ideal solution for epigenome-wide association studies even for FFPE samples.
How does CDNA microarray work?
The principle behind microarrays is that complementary sequences will bind to each other. The unknown DNA molecules are cut into fragments by restriction endonucleases; fluorescent markers are attached to these DNA fragments. These are then allowed to react with probes of the DNA chip.
When did Affymetrix go public?
In 1994, the company’s first product under the “GeneChip” Affymetrix trademark, an HIV genotyping chip was introduced, and the company went public in 1996.
Is DNA methylation associated with altered gene expression in AMD?
DNA methylation is associated with altered gene expression in AMD Invest Ophthalmol Vis Sci. 2012 Apr 24;53(4):2089-105.doi: 10.1167/iovs.11-8449.
How to detect progressive DNA methylation changes in human brain tissue?
Moreover, an IUGR rat model was established by maternal malnutrition method, and gene expression profiles associated with progressive DNA methylation changes in brain tissue were detected using microarray Affymetrix Rat Gene 2.0ST.
How many differentially methylated genes are there between IUGR and Aga 1338?
Based on DNA methylation array, 2265 genes are differentially methylated between IUGR and AGA 1338 genes in the promoter region. Genes with differentially methylated CpG loci in the promoter region enriched in 10 pathways.1311 differentially expressed genes were obtained by Microarray. 36 significantly enriched pathways were identified.