What does quenching mean?
Definition of quench transitive verb. 1a : put out, extinguish. b : to put out the light or fire of quench glowing coals with water. c : to cool (something, such as heated metal) suddenly by immersion (as in oil or water)
What is fluorescence quenching used for?
Fluorescence quenching is an important technique for measuring binding affinity between ligands and proteins. Fluorescence quenching is the decrease in the quantum yield of fluorescence from a fluorophore, induced by a variety of molecular interactions with quencher molecule(s).
What is meant by fluorescence quenching?
Fluorescence quenching refers to any process that decreases the fluorescence intensity of a sample. A variety of molecular interactions can result in quenching. These include excited-state reactions, molecular rearrangements, energy transfer, ground-state complex formation, and colli-sional quenching.
What do you mean by self quenching?
Self-quenching (also called cross-relaxation) occurs between two identical molecules (ions) when a first molecule (or ion) initially in an excited state exchanges energy with the second molecule (ion) that is initially in the ground state, resulting in both molecules (ions) simultaneously changing to excited states …
What is quenching with example?
The process of extinguishing, removing, or diminishing a physical property such as heat or light; e.g., the cooling of a hot metal rapidly by plunging it into water or oil.
What is in quenching oil?
They are composed of base mineral or petroleum oils, and often contain polar lubricants like fats, vegetable oils, and esters, as well as extreme pressure additives such as chlorine, sulfur, and phosphorus. Straight oils provide the best lubrication and the poorest cooling characteristics among quenching fluids.
What is quenching metal?
Quenching involves the rapid cooling of a metal to adjust the mechanical properties of its original state. To perform the quenching process, a metal is heated to a temperature greater than that of normal conditions, typically somewhere above its recrystallization temperature but below its melting temperature.
What is oxygen quenching?
Molecular oxygen is known to be an efficient quencher of the fluorescence of aromatic hydrocarbons (Berlman, 1965; Ware, 1962). The studies so far published show quenching by oxygen to be a diffusion-controlled process in which virtually every collision with the excited fluorophore is effective in quenching.
What does Quenchable mean?
1. To put out (a fire, for example); extinguish. 2. To suppress; squelch: The disapproval of my colleagues quenched my enthusiasm for the plan.