What is the purpose of tetrazolium chloride TTC?

Triphenyl tetrazolium chloride, TTC, or simply tetrazolium chloride (with the formula 2,3,5-triphenyl-2H-tetrazolium chloride) is a redox indicator commonly used in biochemical experiments especially to indicate cellular respiration (example: to check for the viability of seeds).

How does triphenyl tetrazolium chloride work?

TTC is a colorless water-soluble dye that is reduced by the mitochondrial enzyme succinate dehydrogenase of living cells into a water-insoluble, light sensitive compound (formazan) that turns healthy/normal tissue deep red.

What is the required reperfusion period for assessment of myocardial infarct size using triphenyltetrazolium chloride staining in the rat?

60 minutes
For acute assessment of infarct size, reperfusion for 60 minutes or more is optimal because the infarct does appear homogeneous at that time and does not become larger with longer reperfusion periods.

What is a TTC test?

Triphenyl tetrazolium chloride (TTC) dye test for quick diagnosis of urinary tract infection.

What is the role of 2 3 5 triphenyl tetrazolium chloride in measuring dehydrogenase activity?

Dehydrogenase assays based on the reduction of 2,3,5- triphenyltetrazolium chloride (TTC) to the creaming red-coloured formazan (TPF), have been used to determine microbial activity in soil.

Why does TTC turn red?

Tetrazolium red is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions, which subsequently reduce the colorless tetrazolium salt solution to a red compound called formazan. Living cells turn red while dead cells remain colorless.

How do you get 1% in the TTC?

TTC Solution 1% is generally used in a concentration of 0.01 g per 100 mL of broth or agar medium. This concentration is achieved by adding 1 mL TTC Solution 1% to 100 mL sterile medium cooled to 50–60 °C.

How do I prepare for 1% TTC?

What does a positive motility test look like?

Expected Results. Positive: Diffuse, hazy growths that spread throughout the medium rendering it slightly opaque. Negative: Growth that is confined to the stab-line, with sharply defined margins and leaving the surrounding medium clearly transparent.

What is triphenyl formazan?

General description. 1,3,5-Triphenyltetrazolium formazan is an insoluble compound produced upon the reduction of 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt, which is an indicator of cell redox potential. This reaction is irreversible.

Why is TTC not red before bacterial growth?

TTC (2,3,5,-triphenyltetrazolium chloride) is incorporated in the medium to add visual enhancement of bacterial growth. Tetrazolium salt is colorless, but as the organism grows the dye is incorporated into the bacterial cells and reduced to an insoluble red pigment, formazan.

Is triphenyltetrazolium chloride (TTC) a useful staining technique for myocardial infarction?

Comparison of triphenyltetrazolium chloride (TTC) staining versus detection of fibronectin in experimental myocardial infarction Staining with triphenyltetrazolium chloride (TTC), although controversial, has frequently been used for the delineation of myocardial infarction.

Is there a standardized procedure for staining with TTC?

What is the best way to stain the brain for infarcts?

2,3,5-Triphenyltetrazolium chloride (TTC) staining is a convenient procedure for detection of brain infarcts but no standardized procedure is available. We report here an optimized and economic procedure of staining with TTC. Rats were subjected to reversible middle cerebral artery (MCA) occlusion (2-h ischemia and 24-h reperfusion).

Does TTC staining of damaged brain areas after MCA constrict gene and protein analysis?

TTC staining of damaged brain areas after MCA occlusion in the rat does not constrict quantitative gene and protein analyses