What is BD HTS?
The BD High Throughput Sampler (HTS) is a compact, high-speed sample loading device for use with the BD LSR II, BD FACSCanto, and BD FACSCanto II flow cytometers. BD FACSDiva software controls the sample loader, providing automated acquisition of samples from a multiwell plate.
How do you clean HTS Fortessa?
➢ Prepare the cleaning plate with 250 µl FACSClean in A1-A4, 250 µl FACSRinse in B1-B4. ➢ In Diva select “HTS” → “Clean” and follow instruction (the cleaning requires about 10 min). ➢ Perform “HTS” → “Prime” to rinse the HTS with H2O. ➢ Switch the Fortessa to Standby mode.
What is sheath fluid?
Sheath fluid is the solution that runs in a flow cytometer. Once the sheath fluid is running at laminar flow, the cells are injected into the center of the stream, at a slightly higher pressure. The principles of hydrodynamic focusing cause the cells to align, single file in the direction of flow.
Why is sheath fluid used?
Most modern flow cytometers tightly position the sample for optical analysis via hydrodynamic focusing. Here, a carrier fluid called the sheath fluid is used to position the sample of cells into a single file for optical interrogation.
What is meant by Histolysis?
Definition of histolysis : the breakdown of bodily tissues.
What are fluidic devices?
The new technology, termed “Fluidics”, refers to a class of devices and systems which utilize fluids in mo- tion and under pressure to perform a wide variety of sensing, logic, and control functions- without or with a minimum of moving mechanical parts!
What is histolysis and histogenesis?
The process of tissue-destruction is known as ‘histolysis’; the rebuilding process is called ‘histogenesis. ‘
What causes histolysis?
In cells, histolysis may be caused by uracil-DNA degradation. Origin: New Latin, from Greek ‘ιστος (histos) tissue + λυσις (lusis) dissolution from λυειν to loosen, dissolve. Histolysis is associated with metamorphosis as well as other morphological changes.
Is flow cytometry cheap?
Although flow cytometry is the “gold standard” for accurate and automated measurement of CD4 T lymphocytes, the technique is expensive and requires sophisticated equipment as well as trained personnel to perform it.
Where is cytometry used?
Flow cytometry is a laser-based technique used to detect and analyze the chemical and physical characteristics of cells or particles. It is most commonly used to evaluate bone marrow, peripheral blood and other fluids in your body.