How are onion root tip slides prepared?

How are onion root tip slides prepared?

An Alternative Procedure

1. Cut the tip 5 to 8 mm from the tip of the freshly sprouted root.
2. Place the cut tip on a clean microscope slide.
3. Add 2-3 drops of acetocarmine stain to the slide.
4. Warm the slide gently over the alcohol lamp for about one minute.
5. Cover the slide with a cover slip or lens paper.

Why is onion root tip used to demonstrate mitosis in this experiment?

Why use onion roots for viewing mitosis? The roots are easy to grow in large numbers. cells can be observed. The chromosomes can be stained to make them more easily observable.

What method can be used to investigate mitosis in onion garlic root tip?

Use a light microscope to observe, identify and draw root tip meristem cells in different stages of the cell cycle. measure the sizes of some of these cells. calculate the mitotic index for the garlic root tip tissue that they observe.

How do you make onion root tips?

Procedure Of The Experiment

1. Place an onion on a tile.
2. With the help of a sharp blade, carefully snip the dry roots of the onion.
3. Place the bulbs in a beaker containing water to grow the root tips.
4. It may take around 4 to 6 days for the new roots to grow and appear.

What is an onion root tip?

An onion root tip is a rapidly growing part of the onion and thus many cells will be in different stages of mitosis. The onion root tips can be prepared and squashed in a way that allows them to be flattened on a microscopic slide, so that the chromosomes of individual cells can be observed easily.

What characteristics of onion root tip allowed you to identify mitotic cells?

What is fixation of root tips?

Root tips are grown and preserved in acetic ethanol fixative. Fixed root tips can be stored for at least two weeks prior to staining. Treatment with acid and heat is used to break up the cellulose cell wall allowing stain to permeate the tissue and makes it easier to squash the tissue on a microscope slide.

Why would an onion root tip be a good place to study mitosis quizlet?

Why is an onion root tip a good place to look for dividing cells? Rapidly growing part of the onion, indicates many cells will be in different stages of mitosis.