How can I learn pipetting?

How can I learn pipetting?

How good are you?

1. Pre-wet the pipette tip.
2. Immerse the tip to the proper depth during aspiration.
3. Pause consistently after aspiration.
4. Use consistent plunger pressure and speed.
5. Pull the pipette straight out.
6. Examine the tip BEFORE dispensing a sample.
7. Examine the tip AFTER dispensing a sample.
8. Use standard mode pipetting.

What is the difference between Mohr and serological pipettes?

Mohr pipettes: Have hash marks always end before the tip. Serological pipettes: have marks that continue all the way down to the tip. Mohr & Serological Pipette (graduated pipette): They have graduated volumetric markings, and are designed to deliver various volumes with an accuracy of +/- 0.5 -1.0%.

How much do pipettes hold?

These disposable pipets are also called pipettes and work similar to an eye dropper or medicine dropper. Use them for quick, safe transfer of liquids. Each is 6 inches long and the tip is graduated to 1 ml and marked at . 5 ml.

Why should you not use a dirty pipette?

Using a dirty pipet causes too little or contaminated solution to be delivered. Leaving little droplets behind on the walls (except for the small amount in the tip) causes too little solution to be delivered. Forcing the pipet into the bulb usually causes liquid to be sucked into the bulb.

How many types of pipettes are there?

Within pipette calibration there are five widely used grades of pipettes, all of which have specific guidelines and requirements regarding use, testing, maintenance, and measurement. The five grades of pipettes include disposable/transfer, graduated/serological, single-channel, multichannel, and repeat pipette.

What are some common pipette handling errors?

Common pipetting errors include:

• Failure to Pre-Wet Pipette Tip.
• Disregarding Temperature.
• Tip Wiping.
• Choosing the Wrong Pipetting Mode.
• Working Too Quickly.
• Pipetting at an Angle.
• Using the Wrong Pipette Tips.

How do electronic pipettes work?

In the air cushion principle, an air cushion separates the liquid in the tip from the pis- ton inside the pipette. The piston moves the air cushion and the liquid is thus taken up into the pipette tip or dispensed out of it. The air cushion thus works like an elas- tic spring, to which the liquid sticks.