What is the transfection reagent?
Transfection Reagent Basics Transfection is the process of introducing nucleic acids into eukaryotic cells by nonviral methods. Using various chemical or physical methods, this gene transfer method enables the study of gene function and protein expression in a cellular environment.
What are 3T3 cells used for?
NIH/3T3 is a fibroblast cell line that was isolated from a mouse NIH/Swiss embryo. This cell line is highly sensitive to sarcoma virus focus formation and leukemia virus propagation and has proven to be very useful in DNA transfection studies.
What does NIH3T3 stand for?
They were obtained from desegregated NIH Swiss mouse embryo fibroblasts by George Todaro and Howard Green. 3T3 stands for “3-day transfer, inoculum 3×105 cells” and is derived from the original cell transfer and inoculation protocol.
What is 3T3 L1 cells?
3T3-L1 is a fibroblast that was isolated from the embryo of a mouse. This cell line can be used to study the basic cellular mechanisms associated with diabetes, obesity, and related disorders. Animal cells.
What is transfection Class 12?
Transfection refers to the introduction of foreign DNA (genetic material other than host genomes) into the cell. The main purpose of transfection is to alter the host genome to express or block the expression of the protein associated with the gene.
Are 3T3 cells transformed?
NIH 3T3 cells became transformed morphologically when they were cultured in :10% FBS and in 2% or 5% but not in 10% of CS.
Are 3T3 cells immortalized?
The 3T3-L1 line is effectively self-immortalised. It is a line that is immortalised by clonal selection and continuous culture rather than by a transgene such as SV40 large T.
What are 3T3 fibroblasts?
3T3 cells are several cell lines of mouse embryonic fibroblasts. The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green.
What are BALB 3T3 cells?
Cell lines developed from disaggregated BALB/c mouse embryos. They are extremely sensitive to CONTACT INHIBITION, and highly susceptible to transformation by SV40 VIRUS and murine sarcoma virus (SARCOMA VIRUSES, MURINE).
What is 3T3-L1 preadipocytes?
3T3-L1 preadipocytes is the source of mouse embryos, having the morphology of fibroblast cells and the ability to differentiate into mature adipocytes under the stimulation of classic hormonal cocktails, including insulin, dexamethasone, and 3-isobutyl-1-methyl xanthine.