What is a receptor binding assay?

What is a receptor binding assay?

Receptor-binding assays have traditionally been a mainstay of drug development. These assays employ a simple biological preparation of cell membranes and a labeled compound to bind specific protein receptors in those membranes.

What binds to nuclear receptors?

Ligands that bind to and activate nuclear receptors include lipophilic substances such as endogenous hormones, vitamins A and D, and xenobiotic hormones. Because the expression of a large number of genes is regulated by nuclear receptors, ligands that activate these receptors can have profound effects on the organism.

How are nuclear receptors activated?

Nuclear receptors are activated by lipid-soluble signals (e.g., steroid hormones) that cross the plasma membrane. Once activated, most function as transcription factors to control gene expression for numerous biological processes.

What are radioligand binding assays?

Radioligand binding assays provide sensitive and quantitative information about guanine nucleotide protein G protein-coupled receptor (GPCR) expression and affinity for a wide variety of ligands, making them essential for drug structure-activity studies and basic GPCR research.

Do peptide hormones bind to nuclear receptors?

These peptide hormones activate steroid hormone biosynthesis in a limited number of sites, and these ligands for nuclear receptors fînd their way to all cells via the circulation.

Does cortisol bind to nuclear receptors?

The glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is the receptor to which cortisol and other glucocorticoids bind. The GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response.

Is Elisa A ligand binding assay?

Our ligand binding assay lab experts are experienced in the selection of the most suitable assay format, including enzyme-linked immunosorbent assay (ELISA), electrochemiluminescence assays (ECL), or radioimmunoassay (RIA), cell-free, cell-based assay formats, and next generation ligand binding assays (Simoa).