What is reptilase test?
Reptilase time (RT) test is used to evaluate a prolonged TT. Reptilase is a thrombin-like enzyme isolated from the venom of Bothrops atrox. Thrombin splits small fibrinopeptides A and B from fibrinogen molecules, producing fibrin monomer, which polymerizes to form a clot.
What is the function of reptilase in coagulation testing?
The reptilase time measures the rate of fibrin clot formation after the addition of reptilase, a proteolytic enzyme derived from the venom of Bothrops atrox, to citrated plasma.
What is the significance of reptilase time test as an adjunct to thrombin time test?
The reptilase time is independent of thrombin generation disturbances or disturbances in the action of thrombin on fibrinogen. Therefore, it can be used to confirm heparin contamination or to obtain similar information as with the thrombin clotting time in heparinized and hemophiliac patients.
What is a thrombin test?
The thrombin time (TT), also known as the thrombin clotting time (TCT), is a blood test that measures the time it takes for a fibrin clot to form in the plasma of a blood sample. This assesses the activity of fibrinogen and is used to investigate excessive bleeding or inappropriate blood clot formation.
What is reptilase time used for?
Reptilase time (RT) is a blood test used to detect deficiency or abnormalities in fibrinogen, especially in cases of heparin contamination. Reptilase, an enzyme found in the venom of Bothrops snakes, has activity similar to thrombin.
What does dysfibrinogenemia mean?
Dysfibrinogenemia is a coagulation (clotting) disorder characterized by having an abnormal form of fibrinogen.[11855] Fibrinogen is a protein produced by the liver which helps control bleeding by helping blood clots to form.[11856] Having abnormal fibrinogen results in defective clot formation and can cause an …
How is thrombin test done?
Test procedure After separating the plasma from the whole blood by centrifugation, bovine thrombin is added to the sample of plasma. Clot formation is detected optically or mechanically by a coagulation instrument.
What is the reference range for reptilase time?
The reference range for reptilase time depends on the test kit or instrumentation used in the laboratory but is usually below 20 seconds (ie, 15-19 seconds). Healthy infants aged 6 months or younger may have a slightly prolonged reptilase time by 2-3 seconds.
Which test result will be normal in a patient with dysfibrinogenemia?
In liver-associated acquired dysfibrinogenemia, fibrinogen levels are usually normal, as opposed to congenital dysfibrinogenemia, in which fibrinogen levels are low normal to deficient.
What is aPTT used for?
The aPTT test is used to measure and evaluate all the clotting factors of the intrinsic and common pathways of the clotting cascade by measuring the time (in seconds) it takes a clot to form after adding calcium and phospholipid emulsion to a plasma sample.
What does Dysfibrinogenemia mean?
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