What is LSD1 gene?

What is LSD1 gene?

Lysine-specific histone demethylase 1A (LSD1) also known as lysine (K)-specific demethylase 1A (KDM1A) is a protein in humans that is encoded by the KDM1A gene. LSD1 is a flavin-dependent monoamine oxidase, which can demethylate mono- and di-methylated lysines, specifically histone 3, lysines 4 and 9 (H3K4 and H3K9).

What is KDM1A gene?

Entrez Gene Summary for KDM1A Gene This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase.

What is the chromatin modification function of LSD1?

Specifically, LSD1 binds the androgen receptor and promotes androgen-dependent transcription of hormone-responsive genes, enhancing tumor-cell growth: this action is due to a change in its target, the transcription repressive mark monomethyl and dimethyl-lysine 9 in histone H349,50.

What does histone deacetylation do?

Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl functional groups from the lysine residues of both histone and nonhistone proteins. In humans, there are 18 HDAC enzymes that use either zinc- or NAD+-dependent mechanisms to deacetylate acetyl lysine substrates.

What is HDAC1?

Tocris Summary for HDAC1 Gene Histone deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze acetyl group removal from lysine residues in histones and non-histone proteins, causing transcriptional repression. HDACs are usually components of multiprotein complexes.

What is Jumonji C domain?

Jumonji C (JmjC) domain-containing proteins are a diverse superfamily of proteins containing a characteristic, evolutionarily conserved β-barrel structure that normally contains binding sites for Fe(II) and α-ketoglutarate.

What is LSD1 protein?

LSD1, also known as KDM1A (lysine-specific demethylase 1A) or AOF2 (flavin-containing amine oxidase domain-containing protein 2), is an 852 amino acid flavin-dependent monoamine oxidase (MAO) protein of 110 kDa.

What is the substrate for the LSD1 enzyme?

The volume of the reaction was 50 μL (up to 100 μL if the volume of LSD1–CoREST proteins is large), in which 10 μg of mono- or polynucleosomes (EpiCypher) were used as substrates.