How does Alexa Fluor 488 work?

Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

Is Alexa Fluor 488 a fluorophore?

Alexa Fluor® 488 is a green fluorophore commonly used in applications such as immunolabeling, fluorescence microscopy, and flow cytometry.

What are Alexa Fluor antibodies?

Alexa Fluor® 488 secondary antibody is ideal immunofluorescence antibodies to get bright and sensitive immunofluorescent staining in cell and tissue fluorescent microscopy.

What Colour is FITC?

FITC exhibits an excitation maximum at λ = 495 nm and emission maximum at approximately λ = 519 nm. The color of the compound is yellow while the emitted light is green.

What is Alexa Fluor 488 dye?

Alexa Fluor 488 dye | Thermo Fisher Scientific – US Alexa Fluor 488 Dye Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range.

What is an Alexa Fluor® antibody labeling kit?

Molecular Probes® Alexa Fluor® Antibody Labeling Kits provide a convenient means to label small amounts of antibodies with Alexa Fluor® dyes (choice of 10 colors). This kit is optimized for labeling 100 µg of antibody per reaction with green fluorescent Alexa Fluor® 488. Comparably small amounts of other proteins (>40 kDa) can also be labeled.

Can Alexa Fluor® dyes be used without intramolecular quenching?

And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum—Section 1.3.

What are the advantages of probes with high fluorescence quantum yield?