How do you collect Genexpert sputum?
Take a very deep breath and hold the air for 5 seconds. Slowly breathe out. Take another deep breath and cough hard until some sputum comes up into your mouth. Spit the sputum into the plastic cup.
What kind of container is used to collect sputum?
Collection technique: – The patient must be given a labelled sputum container (or a Falcon® tube, if the sample is to be shipped by air). – Have the patient take a deep breath, hold for a few seconds, exhale, repeat two or three times, then cough: sputum is material brought up from the lungs after a productive cough.
Which is the correct procedure for collecting a sputum?
To collect a sputum sample, you should do the following:
- Brush your teeth and rinse your mouth without using antiseptic mouthwash.
- Take a couple of long, deep breaths.
- Breathe deeply again and cough hard until sputum comes up.
- Spit out the sputum into the sample cup.
What is sputum Genexpert?
The Genexpert diagnoses TB by detecting the presence of TB bacteria. The test is a molecular TB test which detects the DNA in TB bacteria. It uses a sputum sample and can give a result in less than 2 hours. The Genexpert can also detect the genetic mutations associated with resistance to the drug Rifampicin.
What is sputum container?
Collection Container Sputum collection containers are available from the physician’s office or from the laboratory. These are sterile and should be opened only when putting in specimen. After the specimen is obtained, replace the lid tightly on the cup and return to the laboratory within two hours.
Which is a rule for collecting specimens?
The first specimen should be collected within seven days of disease onset and the second specimen should be collected two to three weeks later. Proper transport and prompt delivery of specimens to the laboratory are critical for obtaining useful laboratory test results.
What is expectorated sputum?
Expectoration or sputum production is the act of coughing up and spitting out the material produced in the respiratory tract.
When do you collect a sputum specimen?
first wake
Best time of day to collect sputum is when you first wake. Do not eat, drink or smoke before coughing up sputum from the lungs. Rinse (do not swallow) the mouth with water before sputum is collected to minimize residual food particles, mouthwash, or oral drugs that might contaminate the specimen.
What is the difference between GeneXpert and TB PCR?
For Smear positive specimens, Xpert MTB/RIF assay showed 93.8% sensitivity, which was higher than Advansure TB/NTM real-time PCR at 87.5%. On the other hand, for smear negative specimens, Xpert MTB/RIF assay showed 65.5% sensitivity, which was lower than Advansure TB/NTM real-time PCR at 75.9%.
What is the procedure of sputum collection?
The procedure of sputum specimen collection is usually non-invasive. In medicine, it is comparatively simple. However, in some clinical settings, the approach may be more vigorous due to the inability of the patient to expel such fluid from the upper respiratory tract.
How should sputum samples be stored and transported?
Storage and Transport of Sputum Specimens Collection sites should refrigerate samples that cannot be transported immediately to reduce growth of contaminating organisms Specimens should be delivered to the laboratory as soon as possible, within 24 hours of collection is optimal (avoid batching)
What are the characteristics of a good sputum specimen?
Sputum Quality. • Specimens are thick and contain mucoid or mucopurulent material. • Ideally, 3–5 ml in volume, although smaller quantities are acceptable if the quality is satisfactory. • Poor quality specimens are thin and watery. Saliva and nasal secretions are unacceptable.
What is a sputum analysis?
The sputum analysis involves an analytical approach to investigate the cellular and acellular components expelled from the patient’s upper respiratory tract. This procedure is essential in the evaluation and management of lower respiratory infections or other longstanding health conditions.[1]