What is the function of the TATA box?
The TATA box is able to define the direction of transcription and also indicates the DNA strand to be read. Proteins called transcription factors can bind to the TATA box and recruit an enzyme called RNA polymerase, which synthesizes RNA from DNA.
What is eukaryotic gene expression?
Gene expression in eukaryotes is influenced by a wide variety of mechanisms, including the loss, amplification, and rearrangement of genes. Genes are differentially transcribed, and the RNA transcripts are variably utilized. Multigene families regulate the amount, the diversity, and the timing of gene expression.
Why is gene regulation in eukaryotes more complex?
Gene regulation in eukaryotes is more complex than in prokaryotes. This is in part because their genomes are larger and because they encode more genes. For example, the E. coli genome houses about 5,000 genes, compared to around 25,000 genes in humans.
What is our current understanding of the role of the TATA box in eukaryotes?
The TATA box is the binding site of the TATA-binding protein (TBP) and other transcription factors in some eukaryotic genes. Gene transcription by RNA polymerase II depends on the regulation of the core promoter by long-range regulatory elements such as enhancers and silencers.
What are the three stages in gene expression in eukaryotes?
Eukaryotic transcription is carried out in the nucleus of the cell and proceeds in three sequential stages: initiation, elongation, and termination.
What regulates gene expression?
The actions of most factors that regulate gene expression, including transcription factors, long non-coding RNAs, and others, are modulated by the underlying packaging of each eukaryotic gene into chromatin. The relative “openness” of chromatin controls the access of each of these factors to DNA.
Why is gene regulation important in eukaryotes?
Gene regulation is essential for viruses, prokaryotes and eukaryotes as it increases the versatility and adaptability of an organism by allowing the cell to express protein when needed.