What is RNA Ma?
Messenger RNA (mRNA) is a single-stranded RNA molecule that is complementary to one of the DNA strands of a gene. The mRNA is an RNA version of the gene that leaves the cell nucleus and moves to the cytoplasm where proteins are made.
What is seqFISH?
seqFISH (sequential Fluorescence In Situ Hybridization) is a technology that enables the identification of thousands of molecules like RNA, DNA, and proteins directly in single cells with their spatial context preserved.
What is RNA count?
There are two main ways of measuring the expression of a gene, or transcript, or whatever, in RNA-seq data: counts are simply the number of reads overlapping a given feature such as a gene.
What are RNA oligos used for?
Product details. RNA oligos are short, single- or double-stranded synthetic RNA sequences that can be used in nearly any RNA-specific molecular biology application. Using the Oligo Entry ordering tool, you can design your sequences to contain unmodified RNA bases, 2′-O-methyl RNA bases, or chimeric DNA bases.
How does seqFISH work?
In seqFISH, a series of probes are hybridized to each transcript and then removed, in sequential rounds, to generate a unique temporal barcode for each expressed gene. The number of barcodes increases with the number of fluorophores and hybridization rounds.
What is RNAScope?
RNAScope is a commercially available RNA in situ hybridization (ISH) that allows visualization of single RNA molecules in individual cells in a variety of sample types including formalin fixed paraffin-embedded tissue (25).
What is a read count in RNA sequencing?
The simplest approach to quantifying gene expression by RNA-seq is to count the number of reads that map (i.e. align) to each gene (read count) using programs such as HTSeq-count.
What is a read count?
Typically read count is the total number of reads going into the analysis. It could be based off single or multiple sequencing libraries. Also it can be used to describe the number of reads that align to a region of the reference.
What can aptamers do?
Aptamers are single-stranded oligonucleotides that fold into defined architectures and bind to targets such as proteins. In binding proteins they often inhibit protein–protein interactions and thereby may elicit therapeutic effects such as antagonism.
How do oligos work?
This is a method in which the 4 nucleic acids, A, T, C, and G, are added one by one to form a growing chain of nucleotides. They are built on an oligo building block called a phosphoramidite. During these cycles of adding one nucleotide or base to another, the chain grows in the 3′ to 5′ direction.