What is hippocampal slice preparation?
The preparation of hippocampal slices involves two major steps: (1) preparation of equipment, substrates, and media and (2) dissection and slicing of the hippocampus. While there are many similarities, the details of these steps vary depending on whether the goal is to generate acute slices or slice cultures.
What is organotypic?
Organotypic culture is defined as the culture of an organ collected from an organism. It is one method allowing the culture of complex tissues or organs. It allows the preservation of the architecture of the cultured organ and most of its cellular interactions.
What is an acute brain slice?
Definition. Brain slices are ex vivo preparations obtained by serial sectioning of brain tissue, typically from rats or mice. Acute brain slices are kept vital in vitro for time periods between four and, sometimes, more than twenty-four hours and contain a functional brain cell micro-circuitry in situ.
What is the hippocampus?
Hippocampus is a complex brain structure embedded deep into temporal lobe. It has a major role in learning and memory. It is a plastic and vulnerable structure that gets damaged by a variety of stimuli. Studies have shown that it also gets affected in a variety of neurological and psychiatric disorders.
How do you get brain slices?
- Quickly extract the brain. Pour about half (150 ml) of the ice-cold slicing ACSF into the plastic container or large glass Petri dish and oxygenate.
- Isolate the region of interest. With the razor blade, remove the unwanted parts of the brain, rostral and caudal to the region of interest.
- Obtain brain slices.
How are organotypic culture made?
Organotypic cultures consist of sectioned tumour tissue into thin slices, mounted onto porous membranes for mechanical support and incubated in a controlled condition19.
Why do we do cell culture?
Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells, and mutagenesis and carcinogenesis.
Why organotypic hippocampal cultures?
Organotypic hippocampal cultures offer the possibility for easy gene manipulation and precise pharmacological dissociated neurons methods. Here we present a method to prepare and culture hippocampal slices that can be easily adapted to other brain regions. This method allows easy . In addition, slices can be easily
Is there a stable organotypic brain slice culture system using adult hippocampus?
Although organotypic brain tissue culture could be a useful alternative enabling observation of temporal changes induced by various drugs in living brain tissues, a proper method to establish a stable organotypic brain slice culture system using adult (rather than neonatal) hippocampus has been still elusive.
What is the Culture Culture Media for the study of hippocampal slices?
Hippocampal slices prepared from 2-23-day-old neonates were maintained in culture at the interface between air and a culture medium. They were placed on a sterile, transparent and porous membrane and kept in petri dishes in an incubator. No plasma clot or roller drum were used.
How do you maintain a hippocampal slice in culture?
Hippocampal slices prepared from 2-23-day-old neonates were maintained in culture at the interface between air and a culture medium. They were placed on a sterile, transparent and porous membrane and kept in petri dishes in an incubator.