What is CCK 8 reagent?

What is CCK 8 reagent?

CCK-8 is a one-bottle solution; no premixing of components is required. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays.

How does CCK 8 work?

The kit uses a water-soluble tetrazolium salt to quantify the number of live cells by producing an orange formazan dye upon bio-reduction in the presence of an electron carrier. WST-8 / CCK8 solution is added directly to the test cells with no pre-mixing of components required.

How do you analyze MTS assay?

Calculations involved:

1. Subtract the absorbance of the blank wells from all the wells.
2. Divide the absorbance of the wells which have the cells treated with the drug/inhibitor by the average of the absorbances emitted from the cells in the control wells.
3. Multiply the ratio by 100 to give you the viability in %.

What does WST-8 stand for?

CCK-8 (WST-8) is the highest sensitive dye for the cell based assay. Only in CCK-8, continious culture is possible without killing cells. Observation of Cytotoxicity after incubation with each reagent. HeLa cells were incubated with WST-8 (CCK-8), WST-1, or MTS at 24 hours from addition of those reagents.

What is Dojindo CCK 8 used for?

Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. Dojindo’s highly water-soluble tetrazolium salt, WST-8, is reduced by dehydrogenase activities in cells to give a yellow-color formazan dye, which is soluble in the tissue culture media.

What is the electron mediator in WST-8?

The electron mediator, 1-Methoxy PMS, receives electrons from viable cells and transfers the electrons to WST-8 in the culture medium. Since its formazan dye is also highly water soluble, CCK-8 cannot be utilized for cell staining.