How much does a Nucleofector cost?

How much does a Nucleofector cost?

As nucleic acids are delivered straight into the nucleus, nucleofected cells can be ready for analysis after 2–6 hrs of transfection. The bad part about the system is that, it is very expensive ($22,150 (USD) for commercial and about $10,000 (USD) for academic use).

What does a Nucleofector do?

A Nucleofector Device that comprises unique electrical parameters pre-programmed for each optimized cell type, to deliver the substrate directly into the cell nucleus and the cytoplasm.

Is Nucleofector the same as electroporation?

Nucleofection is an electroporation-based transfection method which enables transfer of nucleic acids such as DNA and RNA into cells by applying a specific voltage and reagents.

What is 4D Nucleofector?

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Product Overview. The 4D-Nucleofector X Unit is one of the four functional modules of the 4D-Nucleofector System. It supports Nucleofection of various cell numbers (2 x 104 to 2 x 107) cells in different formats. There are cell type-specific Optimized Protocols or recommendations available in our knowledge database.

How do you Electroporate a cell?

How electroporation works

1. Step 1 : Prepare cells. Prepare cells by suspending in electroporation buffer.
2. Step 2 : Apply electrical pulse. Apply electrical pulse to cells in the presence of specialized buffer and nucleic acids.
3. Step 3 : Return cells to growing conditions.
4. Step 4 : Assay cells.

Is reverse transfection more efficient?

Reverse transfections are faster to perform than forward transfections, and are the method of choice for high-throughput transfection. Optimize transfections as described in Optimizing Transfections, especially if transfecting a mammalian cell line for the first time.

What are the Nucleofector ® kits?

Nucleofector ® Kits, containing dedicated Nucleofector ® Solutions and Supplements. These act as a protective environment for high transfection efficiency and cell viability, while maintaining physiologically relevant cellular conditions.

What is the Nucleofector Technology by Legacy Amaxa?

Since launching the Nucleofector ® Technology by legacy Amaxa, we have introduced several innovations around those three components to expand its application to further cell types, lower and higher cell numbers or different throughputs.

What is the HT Nucleofector system?

The HT Nucleofector ® System is an independent platform offering high-throughput transfection in 384-well format. With an extremely fast plate processing time of one minute and high reproducibility it is the ideal tool for screening applications. Find out more by visiting the HT Nucleofector ® System product page or watching our video tutorial.

What is the ratio of Nucleofector® solution to supplement?

NotePlease make sure that the entire supplement is added to the Nucleofector® Solution. ratio of Nucleofector® Solution to supplement is 4.5:1. Nucleofector® Solution plus 18 µl of supplement to make 100 µl of total reaction volume. – Culture medium: Schneider’s Drosophila Medium [Lonza Cat.

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What is flow electroporation?

MaxCyte’s flow electroporation technology uses an electrical charge to produce the reversible permeability of cell membranes. This allows for the transfer of molecules, such as nucleic acids and proteins, into the cells thereby creating cell therapies.

How does a Nucleofector work?

The Nucleofector Technology uses a specific combination of optimized electrical parameters and cell type-specific solutions which enables transfer of a molecule directly into the cells’ nucleus.

What is Microprojectile method?

Also known as microprojectile bombardment and particle acceleration. The method used to transform cells using small gold or tungsten particles which are coated with DNA and literally shot into the cell. can be used on either tissue culture cells or seedlings (to make chimeric plants) of any species.

What causes electroporation?

Gene electroporation Application of electric pulses of sufficient strength to the cell causes an increase in the trans-membrane potential difference, which provokes the membrane destabilization. Cell membrane permeability is increased and otherwise nonpermeant molecules enter the cell.

What are transfected cells?

Broadly defined, transfection is the process of artificially introducing nucleic acids (DNA or RNA) into cells, utilizing means other than viral infection.

What is electroporation used for?

Electroporation, or electropermeabilization, is a microbiology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, allowing chemicals, drugs, electrode arrays or DNA to be introduced into the cell (also called electrotransfer).

What is lipofectamine transfection?

Lipofectamine™ Transfection Reagent is a value alternative for transfection of plasmid DNA into eukaryotic cells with a simplified protocol. For optimal results in a wider range of plasmid DNA transfection conditions, we recommend Lipofectamine™ LTX Reagent, due to its optimal balance of potency & low-cytotoxicity.

What is the Nucleofector device?

The Nucleofector TM Device is the single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates (e.g., DNA vectors or siRNA oligonucleotides) in low-throughput format.

What is the function of the Nucleofector II?

The Nucleofector II Device is equipped with a hardware safety that immediately detects arc formation at its beginning to protect the cells from damage. After the arc interruption the Nucleofector II resumes program execu- tion.

How do I program my nucleofection™ II device?

Your Nucleofector™ II Device is now ready for program execution (see 2.7.5). 2.7.5 Program execution 1. After you have chosen the correct program, place the closed cuvette filled with 100 µl Nucleofection™ Sample in the cuvette holder (9). For program execution press the “X” button (7). The cuvette carousel (8) closes automatically (see 2.7.2)

Is there an optimization protocol for the Nucleofector TM I/ii/2b device?

For the Nucleofector TM I/II/2b Device, we do not have an optimization protocol for primary cells due to the large number of different cell type specific solutions. Instead, we offer cell group-specific optimization protocols – refer to option B below.