How does the ISE method measure electrolytes?

How does the ISE method measure electrolytes?

There are two methods for measuring serum electrolytes using ISE: direct and indirect. As the name suggests, with direct ISE the patient’s serum sample is brought into direct contact with the electrode surface and the activity of the relevant ion is measured in the plasma water of the serum.

What is the difference between direct and indirect ISE?

Direct ISE is the technique employed in blood gas and most other point-of-care analyzers whereas indirect ISE is used in the majority of biochemistry profiling analyzers sited in central laboratories.

What is ISE blood test?

ISE means ion-selective electrodes. Indirect ISE: Measures on a total plasma sample (or serum) that has been diluted with a large volume of diluent. Requires that the plasma and erythrocytes are separated by centrifugation.

Why is plasma sample especially EDTA could not be used for direct ISE electrolytes machine?

Citrate (blue top) and EDTA (lavender top) cannot be used for chemistry panels because they chelate minerals (e.g. calcium) and interfere with the tests.

What is the use of electrolyte analyzer?

The electrolyte analyser is a device for measuring the electrolytes in the human body. They are primarily used in the quantitative measurement of sodium, potassium, and chloride in whole blood, serum, or plasma.

What units are used to measure electrolytes?

Electrolyte concentrations are similar whether measured in serum or plasma. Values are expressed as mmol/L for sodium, potassium, chloride, and bicarbonate. Magnesium results are often reported as milliequivalents per liter (meq/L) or in mg/dL. Total calcium is usually reported in mg/dL and ionized calcium in mmol/L.

What is ISE electrolyte?

Direct ISE measures the electrolyte activity without sample dilution and actually measures the electrolyte activity in the plasma water (mmol/kg H2O) rather than concentration in the plasma (mmol/L).

Why electrolyte test is done?

An electrolyte panel is often part of a routine blood screening or a comprehensive metabolic panel. The test may also be used to find out if your body has a fluid imbalance or an imbalance in acid and base levels. Electrolytes are usually measured together.

Why is serum electrolyte test done?

They are essential for metabolism, for proper nerve and muscle functioning, for maintenance of proper water balance, and proper blood pH (acid-base balance). The serum electrolyte test includes a group of tests to measure the following electrolytes: Sodium (Na+), Potassium (K+) and Chloride (Cl-).