How do you make 50 ug ml kanamycin?

How do you make 50 ug ml kanamycin?

Kanamycin – The frozen stock solutions of kanamycin are at 50mg/ml in H2O, and are marked with green. The final concentration for LB liquid culture for growing plasmids is 50ug/ml, and for cosmids is 20ug/ml. To obtain 50ug/ml in 100ml of LB, add 100ul stock solution, and to obtain 20ug/ml, add 40ul stock solution.

Is neomycin and kanamycin the same?

In general, neomycin is used in experiments on prokaryotic cells, while G418 is used in eukaryotic experiments. Kanamycin is an aminoglycoside antibiotic that functions by inhibiting translocation of the ribosome which leads to mistranslation.

How do I resuspend kanamycin?

You can dilute 25 mg of kanamycin and dilute with 1 mL of dH2O and finally filter sterilized. Keep your kanamycin stock in -20C/-30C for long term use. Take 20 ul of your kanamycin stock and add with 9980 ul of sterilized dH2O.

How do you make 50 ug mL ampicillin?

Ampicillin : add 1ml ampicillin (at 100mg/ml) per liter of agar to obtain a final concentration of 100ug/ml. Kanamycin : add 1ml kanamycin stock (at 50mg/ml) per liter of agar to obtain a final concentration of 50ug/ml.

How do I make kanamycin 1000x stock?

How do I make kanamycin 1000x stock?

  1. Dissolve 0.5 g of kanamycin into 10 ml of ddH2O.
  2. Filter through a 0.22 µm filter to sterilize.
  3. Aliquot and store at -20°C.
  4. Use at 1:1000 dilution in LB or LB-Agar.

Are carb and AMP the same?

Yes, the antibiotic carbenicillin can be substituted for ampicillin in antibiotic selection plates when E. coli cells such as JM109 cells (Cat.

What is the difference between kanamycin A and B?

Kanamycin A has a hydroxy group at position C2′, whereas kanamycin B possesses an amino group at this position (Fig 1B). Their specific interactions with bacterial ribosomal RNAs to inhibit bacterial protein synthesis have been clearly observed through X-ray structural analysis [2].

What is the brand name for neomycin?

NEOMYCIN/BACITRACIN/POLYMYXIN – TOPICAL (Mycitracin, Neosporin, Triple Antibiotic) side effects, medical uses, and drug interactions.

How do I make 100mg mL?

Weigh 100 mg (0.100 g) of the essential oil into a 100 mL standard volumetric flask. 2. Make up to the mark (that is, the meniscus mark of the stem of standard volumetric flask) with a non-polar solvent e.g. n-hexane or petroleum ether. This gives you 100 mg/100 mL which is equivalent to 1 mg/mL.