What is the principle of the Kirby Bauer test?
In Kirby-Bauer testing, bacteria are placed on a plate of solid growth medium and wafers of antibiotics (white disks, shown) are added to the plate. After allowing the bacteria to grow overnight, areas of clear media surrounding the disks indicate that the antibiotic inhibits bacterial growth.
What is the advantage of the Kirby-Bauer method?
The microbroth dilution method has become more widely used due to its ability to become automated, however, the Kirby Bauer method may be able to detect subpopulations of resistant bacteria that would be visualized as inner colonies in the zone of diffusion.
What is Kirby Bauer test in microbiology?
The purpose of the Kirby-Bauer disk diffusion susceptibility test is to determine the sensitivity or resistance of pathogenic aerobic and facultative anaerobic bacteria to various antimicrobial compounds in order to assist a physician in selecting treatment options for his or her patients.
What are the factors that influence the Kirby-Bauer method?
The disc diffusion techniques most commonly used (the Kirby-Bauer and Stokes’s tests) take account of important factors, including the inoculum density, the composition of the medium, the delay between application of the disc and incubation, the temperature ofincubation, etc., but the resulting zone of inhibition is …
What type of medium is used in the Kirby-Bauer method?
Mueller-Hinton agar
The standard medium for the Kirby-Bauer method of susceptibility testing is Mueller-Hinton agar (MHA) [6].
What is the minimum inhibitory concentration in the Kirby-Bauer method?
minimum inhibitory concentration: This is the lowest concentration of an antimicrobial drug that prevents visible growth of a microorganism after overnight incubation with media.
Is Kirby-Bauer qualitative or quantitative?
A Zone of Inhibition Test, also called a Kirby-Bauer Test, is a qualitative method used clinically to measure antibiotic resistance and industrially to test the ability of solids and textiles to inhibit microbial growth.
What media is used for the Kirby-Bauer method?
The media used in Kirby–Bauer testing must be Mueller–Hinton agar at only 4 mm deep, poured into either 100 mm or 150 mm Petri dishes. The pH level of the agar must be between 7.2 and 7.4.
Why is Mueller-Hinton agar used during the Kirby-Bauer method?
Mueller Hinton agar is a microbiological growth medium that is commonly used for antibiotic susceptibility testing using the Kirby-Bauer disc diffusion method. MHA is recommended for the diffusion of antimicrobial agents impregnated on paper disc through an agar gel as described in CLSI Approved Standard.
What is special about Mueller-Hinton agar that makes it useful for the Kirby-Bauer test quizlet?
What is special about Mueller-Hinton agar that makes it useful for the Kirby-Bauer test? The plates are pH balanced and have uniform depth to insure reliable results. What is the function of oxidase? It is an enzyme that does final electron transfer to oxygen in ETC.