What is the Ouchterlony procedure?
The specific type of immunodiffusion is called Double (Ouchterlony) microimmunodiffusion. The procedure involves adding antigen and antibody to wells in an agarose gel. The antigen and antibody radially diffuse from the wells. Precipitin lines form where antigen meets specific antibody.
What is a immunodiffusion test?
The immunodiffusion (ID) test, also called the Ouchterlony test, allows antigen detection. Immunodiffusion refers to the movement of the antigen or antibody or both antigen and antibody molecules in a diffusion support medium.
What is contained in the precipitin ring?
i) Precipitin Ring A solution containing the antiserum to be investigated is placed in the bottom of a series of clear test tubes (Fig. 7-5A). Solutions containing increasing concentrations of a known antigen are gently layered above the antibody solution.
What are the patterns of identity in Ouchterlony immunodiffusion?
Pattern of identity occurs when the antigens in the two wells are identical and specific for the antibody in the antiserum present in the third well. The concentration of the two antigens been the same, they will diffuse at the same rate resulting in a smooth line of precipitate.
Why is Ouchterlony important?
Ouchterlony double immunodiffusion (also known as passive double immunodiffusion) is an immunological technique used in the detection, identification and quantification of antibodies and antigens, such as immunoglobulins and extractable nuclear antigens.
What are the limitations of precipitin test explain?
Varied techniques have been developed which are still used in these laboratories, but they all suffer from certain inherent defects such as: (1) The period of time taken to carry out a large number of tests; (2) -the possibility of losing’ small quantities of irreplaceable extracts; (3) the comparatively large …
How does a precipitin test work?
It is best known as a means for distinguishing human from other forms of blood. The procedure is briefly to inject an animal (usually a rabbit) repeatedly with a foreign protein; the serum of that animal then gives a precipitate with that protein, but with no other.