What is range in spectrophotometer?

What is range in spectrophotometer?

Different compounds absorb best at different wavelengths. A UV-visible spectrophotometer uses light over the ultraviolet range (185 – 400 nm) and visible range (400 – 700 nm) of the electromagnetic radiation spectrum. Whereas an IR spectrophotometer uses light over the infrared range (700 – 15000 nm).

What does UV spectroscopy tell you?

UV-Vis Spectroscopy (or Spectrophotometry) is a quantitative technique used to measure how much a chemical substance absorbs light. This is done by measuring the intensity of light that passes through a sample with respect to the intensity of light through a reference sample or blank.

How do you handle UV spectrophotometer?

1. Fill the cuvette with the sample.
2. Place the cuvette in the spectrometer in the correct direction.
3. Cover the cuvette to prevent any ambient light.
4. Collect an absorbance spectrum by allowing the instrument to scan through different wavelengths and collect the absorbance.

What is P nitrophenol used for in Spectrophotometry?

The acyl esters of p-nitrophenol are the most commonly used model substrates to study the esterolytic activity of hydrolases. A quantitative detection of the released p-nitrophenolate can be easily carried out at 405–410 nm by spectrophotometry (Schmidt & Bornscheuer, 2005).

What is a u-2900 spectrophotometer?

The U-2900 is a double-beam spectrophotometer offering high performance, ease of use, and reliability. This system can be used in many applications from routine to research.

What are the features of the u-2900?

U-2900 Features • The U-2900 is equipped with a Large Keyboard • Photometric modes include Absorbance, % T, and Concentration • Wavelength range from 190 – 1100nm • Scan Speeds of 10, 100, 200, 400, 800, 1200, 2400 and 3600nm/min (selectable in UV Solutions) • Wavelength Accuracy ±0.3nm and Wavelength Reproducibility ±0.1nm Read more

Can the u-2900 be controlled by a PC?

U-2900 can be controlled by a PC equipped with optional UV Solutions Program. UV Solutions Program provides not only basic functions such as photometry quantitative analysis and wavelength scan but also the capability of data handling as described below. (U-2910 is the model exclusively for operation with a PC.

What is spectrophotometer?

Spectrophotometer mounting a large-size color LCD, which is simpler to use and higher in dependability. High resolution satisfying European Pharmacopoeia (spectral bandpass: 1.5 nm).

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