What is genome-scale metabolic model?
A genome-scale metabolic model (GEM) is a mathematical representation of the metabolism for an organism and it provides extensive gene–reaction–metabolite connectivity via two matrices: the S matrix for associating metabolites to reactions and the rxnGeneMat matrix associating reactions to corresponding enzymes and …
What does genome-scale mean?
Abstract. Genome-scale metabolic models (GEMs) computationally describe gene-protein-reaction associations for entire metabolic genes in an organism, and can be simulated to predict metabolic fluxes for various systems-level metabolic studies.
What is metabolic network analysis?
Metabolic network analysis is a tool for investigating the features that identify the topology of a metabolic network and the relative activities of its individual branches.
What is genome-scale metabolic network reconstruction?
Genome-scale metabolic reconstruction A metabolic reconstruction provides a highly mathematical, structured platform on which to understand the systems biology of metabolic pathways within an organism.
What is genome-scale sequencing?
The analysis of genome-scale sequence data can be defined as the interrogation of a complete set of genetic instructions in a search for individual loci that produce or contribute to a pathological state.
What are the two main components of genome-scale metabolic model reconstruction?
The reconstruction of a metabolic network of an organism with a completed genome sequence consists of the following steps: 1) genome annotation, 2) automated network reconstruction, 3) network refinement, 4) in vitro experimentation, and 5) gap analysis.
What are the two main components of genome scale metabolic model reconstruction?
What is metabolic engineering in biotechnology?
Metabolic engineering is defined as “the improvement of cellular activities by manipulation of enzymatic, transport, and regulatory functions of the cell with the use of recombinant DNA technology” (Bailey, 1991).
What is the major objective of metabolic engineering?
Metabolic engineering applications are driven by a desire to decrease costs, increase yields, increase efficiency, increase consumer acceptability, and use renewable feedstocks to provide a more environmentally friendly process than traditional chemical approaches.