What is difference between ODS and BDS column?
ODS and BDS are two columns used for reverse-phase chromatography. The key difference between ODS and BDS column is that ODS column contains free –OH functional groups, whereas BDS column contains deactivated –OH groups. Moreover, ODS columns have high peak tailing while BDS columns are designed to reduce peak tailing.
How do you separate column chromatography?
Column chromatography separates substances based on differential adsorption of compounds to the adsorbent as the compounds move through the column at different rates which allow them to get separated in fractions.
How are substances separated by column chromatography?
column chromatography, in analytical chemistry, method for separating mixtures of substances in which a liquid or gaseous solution of the mixture is caused to flow through a tube packed with a finely divided solid, which may be coated with an adsorbent liquid, or through a long capillary tube bearing a thin film of …
What is ODS column full form?
Octadecyl-silica (ODS) is the industry standard packing material for HPLC applications.
What is the meaning of ODS column?
An ODS column is filled with a packing of octadecylsilyl groups (ODS groups or C18 groups) chemically bonded to a silica gel carrier. ODS columns are used for reverse-phase chromatography. ODS columns find a wide range of applications due to their high theoretical plate number and rapid equilibration.
What is column separation?
Column separation refers to the breaking of liquid columns in fully filled pipelines. This may occur in a water-hammer event when the pressure in a pipeline drops to the vapor pressure at specific locations such as closed ends, high points or knees (changes in pipe slope).
What mixtures can be separated by column chromatography?
1 Answer. It is possible to separate different solutes which are dissolved in a solvent. For example, column chromatography can be used to separate different water soluble proteins.
What is separating column?
From Wikipedia, the free encyclopedia. Separating column may refer to: A fractionating column. A column containing ion-exchange resin.
What is end capped column?
In chromatography, endcapping refers to the replacement of accessible silanol groups in a bonded stationary phase by trimethylsilyl groups. End-capped columns have much lower residual silanol group activity compared to non-endcapped columns.