How we can determine antibiotic sensitivity test for Staphylococcus aureus?

How we can determine antibiotic sensitivity test for Staphylococcus aureus?

All isolates confirmed to be S. aureus by PCR were subjected to antibiotic susceptibility testing to oxacillin (5 μg) [37] by disc diffusion test as well as growth on Brilliance MRSA II agar [38], to determine phenotypic resistance to methicillin. Inoculated plates were incubated at 37 °C for 24 h [38].

Does Staphylococcus aureus have antibiotic resistance?

Staphylococcus aureus is notorious for its ability to become resistant to antibiotics. Infections caused by antibiotic-resistant strains often occur in epidemic waves initiated by one or a few successful clones.

What is the antibiotic used for susceptibility testing to differentiate Staphylococcus species?

Antibiotic susceptibility testing showed varying degree of resistance by different staphylococcal species (Table 2). Maximum resistance was observed to penicillin, oxacillin and ciprofloxacin. S. aureus showed higher resistance to penicillin (80%), oxacillin (53%), augmentin (54%) and ciprofloxacin (85%).

What is a cefoxitin screen test?

Cefoxitin is a potent inducer of the mecA regulatory system. It is being recommended for detection of methicillin resistance in Staphylococcus aureus (MRSA) when using disk diffusion testing.

How does Staphylococcus aureus develop antibiotic resistance?

Staphylococcus aureus can become drug-resistant by genetic mutations that alter the target DNA gyrase or reduce outer membrane proteins, thereby reducing drug accumulation (Kime et al., 2019; Yang et al., 2019).

Is Staphylococcus aureus sensitive to streptomycin?

Antibiotic susceptibility of S. aureus isolates, the percentage of isolates resistant to Oxacillin (OX), Ampicillin (AMP), Ciprofloxacin (CIP), Tetracycline (TET), Streptomycin (SMN), Erythromycin, (ERY) and Sulfamethoxazole/Trimethoprim (SXT) was 17, 13, 9, 28, 89, 13 and 11% respectively.

Is Staphylococcus aureus sensitive to Vancomycin?

Methicillin-resistant Staphylococcus aureus (S. aureus) (MRSA), resistant to all antibiotics including Vancomycin, has been reported in Japan, USA, Canada and Brazil.