What is the mechanism of ion exchange resin?
What are ion exchange resins? Ion exchange is a reversible chemical reaction where dissolved ions are removed from solution and replaced with other ions of the same or similar electrical charge. Not a chemical reactant in and of itself, IX resin is instead a physical medium that facilitates ion exchange reactions.
Why is salt used in ion exchange chromatography?
A salt gradient is used to elute separated proteins. At low salt concentrations, proteins having few charged groups are eluted and at higher salt concentrations, proteins with several charged groups are eluted. Unwanted proteins and impurities are removed by washing the column.
How does a Demineralizer work?
Demineralizers or ion exchangers are equipments which can hold ion exchange resins. Demineralization or ion exchange refers to exchange of ions between a solid substance (called a resin) and an aqueous solution (makeup water).
What is resin in ion exchange chromatography?
Ion exchange chromatography resins are composed of positively or negatively charged functional groups that are covalently bound to a solid matrix. Common matrices are cellulose, agarose, polymethacrylate, polystyrene, and polyacrylamide. The latter three matrices allow higher flow rates.
How does ion exchange chromatography work?
Ion-exchange chromatography (IEX) separates proteins (or any biomolecules) based on differences in their net charge at a particular pH. Protein charge depends on the number and type of ionizable amino acid side chain groups.
What does salt do in chromatography?
ION-EXCHANGE CHROMATOGRAPHY. Charged proteins bind to the oppositely charged resins via electrostatic interactions in IEC. Salts, due to its nonspecific effects, modulate binding affinity (binding constant) of the protein. While any salts can be used as a source of ions, NaCl is typically used.
What salt is used in ion exchange chromatography?
NaCl dissociates in Na+ and Cl- in solution. In anion-exchange chromatography, Cl- competes with negatively charged analytes to bind with positively charged stationary phase, thereby eluting the analytes.
What is the pH of DM water?
Pure water by definition is slightly acidic and distilled water will test out around pH 5.8. The reason is that distilled water dissolves carbon dioxide from the air….pH-value.
How does mixed resin work?
Mixed bed resins use SAC and SBA resins combined together. In order to produce deionized water, cation resin is regenerated with Hydrochloric Acid (HCl). The Hydrogen (H+) is positively charged and therefore attaches itself to the negatively charged cation resin bead.
What is resin in chromatography?
What are chromatography resins? Chromatography resins are media used to capture and polish mAbs, antibody fragments, vaccines, and other biomolecules using a stationary phase. There are several resin types for both analytical and purification purposes.