What is Prussian blue formula?

What is Prussian blue formula?

Fe4[Fe(CN)6]3Prussian blue / Formula

How do you make a Prussian blue solution?

Procedure:

1. Deparaffinize and hydrate sections to distilled water.
2. Mix equal parts of hydrochloric acid and potassium ferrocyanide prepared immediately before use.
3. Wash in distilled water, 3 changes.
4. Counterstain with nuclear fast red for 5 minutes.
5. Rinse twice in distilled water.

How do you make Prussian blue stain?

Method

1. Take test and control sections to distilled water.
2. Stain sections in bathophenanthroline solution for 2 hours at room temperature.
3. Rinse well in distilled water.
4. Counterstain in 0.5% aqueous methylene blue for 2 minutes.
5. Rinse well in distilled water.
6. Stand slides on end until completely dry.

How much is Prussian blue?

For cesium poisoning: Adults and teenagers—3 grams (6 capsules) three times a day. Children 2 to 12 years of age—1 gram (2 capsules) three times a day. Children younger than 2 years of age—Use and dose must be determined by your doctor.

What makes Prussian blue blue?

Prussian blue owes its colour to the presence of iron in two different valency states, allowing electrons to move from one orbit to another with great ease and giving rise to very strong absorption in the orange/red part of electromagnetic wavelengths, resulting in a strong reddish blue colour.

What color is Prussian blue?

dark blue colour
Prussian blue, also known as Berlin blue, is a dark blue colour that is artificially made. It is one of the first pigments made synthetically. It was accidentally found in 1704 by two chemists in Berlin. The dark blue uniforms of the Prussian army were dyed this colour.

How does Prussian blue staining work?

PRINCIPLE: The reaction occurs with the treatment of sections in acid solutions of ferrocyanides. Any ferric ion (+3) in the tissue combines with the ferrocyanide and results in the formation of a bright blue pigment called ‘Prussian blue” or ferric ferrocyanide.

What is Prussian blue made of?

Prussian blue, any of several deep-blue pigments that are composed of complex iron cyanides and hence called iron blues. The most common of these pigments are Prussian, Chinese, Milori, and toning blue.

What is Perls reaction?

In Perls’ Prussian or Berlin blue reaction (Max Perls, 1843–81), the section is treated with dilute hydrochloric acid to release ferric ions from binding proteins. These ions will react with potassium ferrocyanide to produce an insoluble blue compound.

What type of stain is Prussian blue?

The stain is an important histochemical stain used to demonstrate the distribution and amount of iron deposits in liver tissue, often in the form of a biopsy. Perls’s procedure may be used to identify excess iron deposits such as hemosiderin deposits (hemosiderosis) and in conditions such as hereditary hemochromatosis.

What is the chemical name of Prussian blue?

iron(III) hexacyanoferrate(II)
Thus, the formula that gives a truer idea of its composition is Fe4[Fe(CN)6]3. Its formal name is iron(III) hexacyanoferrate(II). As shown in the two left-hand drawings, the Fe(CN)6 anion in Prussian blue is octahedral.