What is enzyme kinetics answer?
Kinetics is the study of reaction rates and how they are changed in response to any environmental factors. The key factor which affects the rate of reaction catalyzed by an enzyme is the amount of substrate present [S], the effect of V0 (initial velocity).
What is enzyme kinetics study?
Enzyme kinetics is the study of the binding affinities of substrates and inhibitors and the maximal catalytic rates that can be achieved.
What is the use of enzyme kinetics?
In pharmaceutical sciences, the applications of enzyme kinetics range from hit finding efforts for new chemical entities on a pharmacological target to concentration effect relationships to large-scale biosynthesis. The study of the science of drug metabolism has two principal concepts-rate and extent.
How is enzyme kinetics measured?
The rates of these reactions can be accurately measured using a UV-Visible spectrophotometer. When an enzyme (E) binds with a substrate (S), an intermediate or enzyme/substrate complex (ES) is produced, which can further react and yield a by-product (P), shown in Scheme 1.
Why is understanding enzymes important?
Enzymes are proteins that help speed up chemical reactions in our bodies. Enzymes are essential for digestion, liver function and much more. Too much or too little of a certain enzyme can cause health problems. Enzymes in our blood can also help healthcare providers check for injuries and diseases.
Why are kinetics important?
One reason for the importance of kinetics is that it provides evidence for the mechanisms of chemical processes. Besides being of intrinsic scientific interest, knowledge of reaction mechanisms is of practical use in deciding what is the most effective way of causing a reaction to occur.
Why is kinetics important in biology?
Kinetics are important in biological systems, however because enzymes make reversible many otherwise kinetically irreversible reactions. The rate at which a reaction will proceed (measured by k) is directly related to the amount of energy that must be supplied before reactants and products can be interconverted.
What is the kinetic assay?
An enzyme-based assay that measures the amount of substrate present by correlation of the rate of reaction with the known dependence of the rate on substrate concentration, usually under first-order conditions. ( see also end-point assay)