What does acid phosphatase do in wheat germ?
Introduction. Phosphatases catalyze the hydrolysis of phosphoric-monoester compounds to produce a free inorganic phosphate and an alcohol.
What is the Km and Vmax of acid phosphatase?
The phosphatase had a Km of 0.24 mM and a Vmax of 2.76 μmol/min/mg with PLP.
What class of enzyme does wheat germ acid phosphatase belong to?
Acid phosphatases (APs) are a family of enzymes that belong to the hydrolase class.
What is the optimum pH for wheat germ acid phosphatase?
4.0–7.0
The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.0–7.0 and the optimal temperature is 45 °C.
Where is acid phosphatase found?
the prostate gland
Acid phosphatase is one of a group of enzymes located primarily in the prostate gland and prostatic secretions. Smaller amounts are found in the bone marrow, spleen, liver, kidneys, and blood components such as erythrocytes and platelets.
What is the km of phosphatase?
Alkaline phosphatase has a Km of 8.4 x 10−4.
What do the values Vmax and Km represent in terms of Characterising the acid phosphatase being used?
Under the conditions of this study, the Km refers to the apparent Km for the enzyme activity, or the concentration of substrate at which activity is one half the maximal velocity and Vmax refers to the apparent maximal velocity for enzyme activity, which is the maximum rate of P- hydrolysis.
What is the purpose of acid phosphatase?
Function. Acid phosphatase (ACP, EC number 3.1. 3.2) is an enzyme which removes phosphate from other molecules during digestion. It catalyzes the conversion of orthophosphoric monoester and H2O to alcohol and phosphoric acid.
Why do Enzymologists determine specific activity?
Enzymologists determine specific activity in order to determine the purity of the enzyme sample mixture. As the enzyme becomes more pure in a mixture, the specific activity value increases.
Is acid phosphatase a protein?
Based on this we can distinguish the histidine acid phosphatases (Van Etten, Ann N Y Acad Sci 390:27-51, 1982), the low molecular weight protein tyrosine acid phosphatases and the metal-ion dependent phosphatases.