How do you calculate persistence length?
Calculation of Persistence Length. 〈 r i ⋅ r i + s 〉 = l 2 〈 cos θ ( s ) 〉 = l 2 exp ( − s l / l p ) . Here, s is the chemical distance between two bonds, and l is the bond length.
What is persistent length of DNA?
DNA is among the stiffest of known polymers with a persistence length of ∼50 nm (150 bp) in 0.1 M aqueous NaCl.
How is Kuhn length measured?
For worm-like chain, Kuhn length equals two times the persistence length.
What is persistence length of microtubules?
The mechanical properties of microtubules have therefore been studied intensively for over a decade (1–7). The consensus was that microtubules can be characterized by a persistence length of 4–8 mm (1,6).
What is the persistence length of single stranded DNA?
As is known, the persistence length and the length per base of dsDNA is 50 nm [46] and 0.34 nm [47] respectively. The persistence length of single-stranded DNA, as a kind of flexible polymer, cannot be larger than 50 nm.
What is the length of a polymer chain?
In polymer chemistry the kinetic chain length of a polymer, ν, is the average number of units called monomers added to a growing chain during chain-growth polymerization. During this process, a polymer chain is formed when monomers are bonded together to form long chains known as polymers.
How do you find the contour length of a polymer?
To get the chain contour length, divide MW of the polymer by the MW of the monomer unit and multiply by the length of the monomer unit.
How is DNA length measured?
The structure of DNA is such that between two consecutive base pairs, a distance of 0.34 nm (0.34×10−9 m) is present. Thus, one can find the total length of a DNA of an organism by multiplying the total number of base pairs with a distance between two consecutive base pairs.
What is the length of double stranded DNA?
As is known, the persistence length and the length per base of dsDNA is 50 nm [46] and 0.34 nm [47] respectively.
What is the persistence length in physics?
Persistence length. The persistence length is considered to be one half of the Kuhn length, the length of hypothetical segments that the chain can be considered as freely joined. The persistence length equals the average projection of the end-to-end vector on the tangent to the chain contour at a chain end in the limit…
What is the persistence length of a chain?
The persistence length is considered to be one half of the Kuhn length, the length of hypothetical segments that the chain can be considered as freely joined. The persistence length equals the average projection of the end-to-end vector on the tangent to the chain contour at a chain end in the limit of infinite chain length.
What is the persistence length of DNA?
The persistence length is the characteristic length scale of that exponential decay. For the case of a single molecule of DNA the persistence length can be measured using optical tweezers and atomic force microscopy. Persistence length measurement of single stranded DNA is viable by various tools.
What is the difference between beam theory and persistence length theory?
Informally, for pieces of the polymer that are shorter than the persistence length, the molecule behaves rather like a flexible elastic rod/beam (beam theory), while for pieces of the polymer that are much longer than the persistence length, the properties can only be described statistically, like a three-dimensional random walk.