What is VAV cre?
Vav-Cre transgenic mice express Cre recombinase under the control of the human vav 1 oncogene (VAV1) regulatory elements in all nucleated blood cells as well as hematopoietic stem cells. This strain may be useful as a Cre-lox tool for deletion of floxed sequences in cells of the hematopoietic system.
How does Mx1 CRE work?
The Cre recombinase is under the control of the Mx1 promoter, which can be induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA (such as poly I:C); enabling the “knockout” at any time during development in progeny of a floxed mutant mated to …
Do neutrophils express LysM?
LysM-cre promotes significant deletion in macrophages, but also in neutrophils. Like LysM-cre, GE-cre promotes significant deletion in neutrophils and macrophages. F4/80-cre:ROSA-EYFP mice show ~50% deletion in peritoneal macrophages, but do not exhibit deletion in other types of macrophages.
How does Cre loxP work?
Concerning the mechanism of Cre-loxP system, a single Cre recombinase recognizes two directly repeated loxP site, then the Cre excises the loxP flanked (floxed) DNA, thus creating two types of DNA with circular, excised and inactivated gene Y (Figure 1A).
Are murine VAV gene regulatory elements useful for CRE transgenic mice?
In these animals, a Cre transgene is expressed under control of murine vav gene regulatory elements. To assess their usefulness, vavCre transgenic mice were bred with R26R mice, which express a lacZ reporter gene only in cells where Cre-mediated recombination has occurred.
How useful are vavcre transgenic mice for studying Cre-mediated recombination?
To assess their usefulness, vavCre transgenic mice were bred with R26R mice, which express a lacZ reporter gene only in cells where Cre-mediated recombination has occurred. VavCre/R26R double-heterozygous offspring were analyzed by beta-galactosidase histochemistry and flow cytometry.
What is vavcre mutagenesis?
VavCre transgenic mice: a tool for mutagenesis in hematopoietic and endothelial lineages Cre transgenic mice can be used to delete gene sequences flanked by loxP sites in specific somatic tissues. We have generated vavCre transgenic mice, which can be used to inactivate genes specifically in adult hematopoietic and endothelial cells.
What is the best way to target specific Cre recombinant genes?
Conditional gene targeting, based on Cre-lox or other systems, requires frequent genotyping of transgenic mouse populations and monitoring of tissue-specific Cre recombinatory efficiency. This is currently achieved by Southern analysis from tail- and tissue-derived DNA.