What is toluidine blue stain used for?

What is toluidine blue stain used for?

Toluidine blue has been used in vivo to identify dysplasia and carcinoma of the oral cavity. Use of toluidine blue in tissue sections is done with the aim to highlight components, such as mast cells granules, mucins, and cartilage.

What is toluidine blue test?

Summary. Toluidine blue stain is used as a marker to differentiate lesions at high risk of progression in order to improve early diagnosis of oropharyngeal carcinomas. This study focused on 45 oral mucosal lesions in 32 patients (13 female, 19 male). In 9 cases, multiple biopsies were collected.

How do you make toluidine blue stain?

Procedure:

1. Deparaffinize and hydrate sections to distilled water.
2. Stain sections in toluidine blue working solution for 2-3 minutes.
3. Wash in distilled water, 3 changes.
4. Dehydrate quickly through 95% and 2 changes of 100% alcohol (10 dips each since stain fades quickly in alcohol).

Why stains are given in the night?

To maximize the effects of statins with a short half-life, fluvastatin, lovastatin, and simvastatin should be dosed at bedtime allowing the greatest drug concentration to be present during peak endogenous cholesterol synthesis.

What causes Submucous fibrosis?

Oral submucous fibrosis (OSF) is a premalignant condition caused by betel chewing. It is very common in Southeast Asia but has started to spread to Europe and North America. OSF can lead to squamous cell carcinoma, a risk that is further increased by concomitant tobacco consumption.

Is methylene blue gram positive?

The predominant colour in gram staining is methylene blue. Gram-positive organisms are organisms that preserve the primary colour and look purple-brown under a microscope.

When should you take a statin the answer just changed?

Those people who have a greater than 7.5% risk of a heart attack over that period should take a cholesterol-lowering drug in the statin group, such as atorvastatin, simvastatin, or pravastatin. You can read the new guidelines here, and you can calculate your risk here.

What is toluidine blue O (TBO)?

Visit Product Comparison Guide Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. It is a metachromatic dye suitable for a wide variety of histological staining procedures.

What is toluidine blue dye made of?

The dye listed as simply Toluidine Blue, Product 89640, is the zinc chloride double salt. For routine use today, the chloride form should be utilized. The product number recommended for biological/histological staining is Product 198161, Toluidine Blue O.

Is toluidine blue zinc chloride or ZnCl?

There is a chloride form and zinc chloride double salt. Products T3260 and 198161 are the chloride form. This is the form certified by the Biological Stain Commission. The dye listed as simply Toluidine Blue, Product 89640, is the zinc chloride double salt.

What is TBO dye used for?

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite.