What is the function of alpha actinin?
α-actinin localization and function α-actinin primarily influences the cohesiveness and mechanics of the cytoskeleton by cross-linking actin filaments and other cytoskeleton components to create a scaffold that imparts stability and forms a bridge between the cytoskeleton and signaling pathways.
What does alpha actinin bind to?
α-Actinin binds β1, β2, and β3 integrins. The binding sites for α-actinin have been localized to the membrane-proximal half of the β1 or β2 integrin cytoplasmic domain (Liu et al., 2000). α-Actinin competes with talin in early adhesions by linking integrin and actin (Roca-Cusachs et al., 2013).
How do you stain cardiomyocytes?
Staining cardiomyocytes Collect the cells by centrifugation at 100 x g for 1 min and add 5 mL of permeabilization solution (e.g., 0.5% Triton X-100 in PBS). Incubate for 20 min at room temperature on rocker.
Does alpha actinin transfer force?
α-actinin is involved in force transmission to the ECM via integrin binding21, while zyxin is important in maintenance of stress fiber integrity under applied loads22.
Where is alpha actinin found?
In non-muscle cells, alpha-actinin is found along the actin filaments and in adhesion sites. In striated, cardiac and smooth muscle cells, it is localized at the Z-disk and analogous dense bodies, where it forms a lattice-like structure and stabilizes the muscle contractile apparatus.
Is Nebulin a contractile protein?
Nebulin, a component of the contractile apparatus in skeletal muscles, is a giant protein (up to 900 kD in size), whose purpose is poorly understood. In a paper appearing this month in JGP, Kawai et al. explain what nebulin does in muscle fibers, and why it is so important in muscle function (1).
Where is Fimbrin found?
I-fimbrin is expressed by intestine and kidney epithelial cells. T-fimbrin is found in epithelial and mesenchymal cells derived from solid tissue where it does not become phosphorylated.
What is ACTN3 deficiency?
α-Actinin-3 deficiency results in altered skeletal muscle metabolism, with decreased glycogen phosphorylase activity and a shift in the metabolic profile of fast glycolytic fibers towards a ‘slower’ oxidative phenotype without a change in fiber type.
What does nebulin do in the sarcomere?
Nebulin binds polymeric actin and acts as a template protein for the thin filament in the skeletal muscle sarcomere (Jin and Wang 1991a, b).
What is nebulin used for?
Because its length is proportional to thin filament length, it is believed that nebulin acts as a thin filament “ruler” and regulates thin filament length during sarcomere assembly and acts as the coats the actin filament.
What are Alpha actinins?
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules.
How do you test for alpha actinin in mouse heart tissue?
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66895-1-Ig (alpha Actinin antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Is aliquoting necessary for-20oC storage of Alpha actinins?
Aliquoting is unnecessary for -20oC storage. Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules.
Does 66895-1-ig target alpha actinin?
Alpha Actinin primary antibody included. 66895-1-Ig targets Alpha Actinin in WB, IHC applications and shows reactivity with Human, rat, mouse samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3.