What is the advantages of using the Thermofisher kit MagMAX?
Benefits of MagMAX magnetic bead-based purification: Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples.
How does MagMAX work?
Samples are disrupted using zirconia beads in a guanidinium thiocyanate–based solution; this rapidly releases nucleic acid while simultaneously inactivating nucleases. Samples are then diluted with isopropanol, and paramagnetic beads are added to the sample.
What is a viral kit?
The PureLink® Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants.
What is nucleic acid where it located?
Although first discovered within the nucleus of eukaryotic cells, nucleic acids are now known to be found in all life forms including within bacteria, archaea, mitochondria, chloroplasts, and viruses (There is debate as to whether viruses are living or non-living).
What is total nucleic acid?
The Aridia Total Nucleic Acid Isolation Kit is intended to be used by professionals trained in nucleic acid techniques to isolate viral and cellular nucleic acids (DNA and RNA) from clinical samples. The isolated nucleic acids can be used for PCR amplification or other molecular biology applications.
What is RNA carrier?
Poly (A) carrier RNA is used for quantitative precipitation/purification of RNA and DNA. It improves recovery of short fragments (< 200 bp) or low amounts of nucleic acids. Chain length: 2100 to 10000 nucleotides.
What foods have nucleic acids?
Nucleic acids are found in all living things, including the foods you eat. Based on current research, meat, fish, seafood, legumes, and mushrooms contain the highest levels of these compounds.
What 3 things make up nucleic acids?
Nucleic acids are giant biomolecules made of monomers called nucleotides. Nucleotides have three components: pentose sugar (5-carbon sugar), phosphate group, and nitrogenous base.
How do you isolate viral DNA?
Compared to RNA viruses, DNA viruses (e.g., HBV) are usually more difficult to isolate and require a digestion of samples with Proteinase K which is provided in the kit. Lysis buffer and ethanol create appropriate conditions for binding of nucleic acids to the silica membrane of the NucleoSpin® Virus Binding Strips.
What is total nucleic acid extraction?
Total nucleic acid extraction products purify both RNA and DNA from samples. During DNA and RNA extraction, samples all progress though a common set of steps involving cell lysis, clearing, inactivation of nucleases, nucleic acid binding, washing and elution.