What is allele-specific expression?
Allele-specific expression (ASE) refers to the characteristic of preferentially expressing a parental allele in the hybrid due to variations in regulatory sequences from the parental genomes (11). The expression difference caused by ASE may lead to phenotypic variation depending on the function of the genes.
Is an allele an expression of a gene?
The two chromosomal copies (alleles) of a gene are designated A and a. In most cases, both alleles are transcribed; this is known as bi-allelic expression (left). However, a minority of genes show monoallelic expression (right). In these cases, only one allele of a gene is expressed (right).
What is allele-specific amplification?
Allele-specific polymerase chain reaction (AS-PCR), also known as amplification refractory mutation system (ARMS) or PCR amplification of specific alleles (PASA) is a PCR-based method which can be employed to detect the known SNPs [7]. The concept of AS-PCR was initiated by Newton et al.
How does allele-specific PCR work?
ASPCR works because an oligonucleotide primer that forms a 3′ mismatch with the DNA template will be refractory to primer extension by Thermus aquaticus DNA polymerase. Therefore, oligonucleotide primers specific for all known alleles can be synthesized and used to detect the alleles in DNAs of unknown genotype.
What causes allele-specific expression?
What is the expressed allele called?
Phenotypes (the expressed characteristics) associated with a certain allele can sometimes be dominant or recessive, but often they are neither.
When an allele is phenotypically expressed it is a recessive allele?
Key terms
| Term | Meaning |
|---|---|
| Dominant allele | Allele that is phenotypically expressed over another allele |
| Recessive allele | Allele that is only expressed in absence of a dominant allele |
| Homozygous | Having two identical alleles for a particular gene |
| Heterozygous | Having two different alleles for a particular gene |
What is allele specific PCR used for?
Allele-specific polymerase chain reaction (AS-PCR) is a technique based on allele-specific primers, which can be used to analyze single nucleotide polymorphism (SNP) effectively including the transition, transversion and insertion/deletion polymorphism and has been exploited in the study of diseases research, molecular …
How do you make allele specific primers?
Allele Specific Primer Design Guidelines
- ASPE primers should be from the same DNA strand (per target sequence) and be synthesized for all the sequence variants.
- The Ta opt should be around 51-56 °C.
- The SNP should be present at the 3′ end of the allele specific primer for accurate hybridization.