What is a chromatography column made of?
Materials. Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer, stainless steel or ceramic net is usually applied.
Which lamp is used in HPLC?
HERAEUS NOBLELIGHT DEUTERIUM LAMPS Deuterium lamps emit an almost continuous spectrum of light ranging from the main UV wavelengths of 160 – 400 nm to the visible spectral range (800 nm). This makes them the ideal light source for high precision absorption measurements, e.g. in HPLC.
Is UV detector used in HPLC?
UV detector is a very commonly used detector for HPLC analysis. During the analysis, sample goes through a clear color-less glass cell, called flow cell. When UV light is irradiated on the flow cell, sample absorbs a part of UV light.
What is universal detector in HPLC?
A universal detector is defined as the one which ‘can respond to every component in the column effluent except the mobile phase’2. In contrast, selective detectors are defined as ‘detectors which respond to a related group of sample components in the column effluent’.
What does AU mean in HPLC?
The x axis represents retention time, and AU on the y axis represents absorbance units (a signal corresponding to the response created by the detector) at 210 nm.
What is Dad detector?
Diode-Array Detection (DAD) or Photodiode-Array Detection (PDA) is an analytical technique that can be used to determine the purity of an analyte or related impurity peak eluting during an HPLC separation.
What is BPG chromatography column?
BPG chromatography columns Glass chromatography columns for process development or biopharmaceutical manufacture, designed for hygienic operation and easy, efficient packing.
What bpg kit should I use for my bpg 100 column?
BPG 100, Accessories for Packing the column, parts identification. For flow rates 3 l/h-175 l/h use Low Flow Kit 28-9301-82. For flow rates 7.5 l/h-510 l/h use High Flow Kit 28-9301-83.
Are bpg columns suitable for use with äktaprocess?
Suitable systems: BPG columns are designed for use with ÄKTAprocess systems and have design pressures of 8 bar (BPG 100), 6 bar (BPG 140 and 200), 4 bar (BPG 300) and 2.5 bar (BPG 450). There are no available product features to display for this product variant. No reviews have been left for this product.
How many exchangeable tube lengths are available for BPG columns?
Three standard exchangeable tube lengths are available for BPG columns. Note that you must also replace the tube rods when changing the column tube. What type of pump do I need for my column? Packing and running packed bed columns requires a pump that gives a pulse-free flow. Installing a pulse reducer may also be necessary.