What does MitoTracker green measure?

What does MitoTracker green measure?

MitoTracker green (MTG) and nonylacridine orange (NAO) are fluorescent probes which have been commonly used to assess mitochondrial mass. This is based on the assumption that both MTG and NAO accumulate in mitochondria regardless of the mitochondrial transmembrane potential (ΔΨm).

What does MitoTracker green stain?

MitoTracker Green FM is green-fluorescent mitochondrial stain which appears to localize to mitochondria regardless of mitochondrial membrane potential. The dye will stain live cells but is not well-retained after aldehyde fixation.

Is MitoTracker green fixable?

Most of the MitoTracker® dyes are well-retained following fixation and permeabilization using the protocol described below. However, MitoTracker® Green FM and MitoTracker® Red FM are not retained well after fixation. 2.1 Washing the cells.

How do you use a MitoTracker?

Protocol

1. Grow bloodstream form T.
2. Prepare a 1 mM stock of Mitotracker (Invitrogen/Molecular Probes, #M7512) in DMSO, i.e. 50 μg CMXROS in 94 μl.
3. Add 1 μl of the stock Mitotracker solution to a 10ml culture, giving a final concentration of 100 nM, and incubate at 37oC for 5 minutes.

What does MitoTracker Red measure?

It is a specific dye called “MitoTracker Red CMXRos”. It is special dye to measure mithocondiral membrane potential (MMP).

How does MitoTracker orange work?

MitoTracker® Orange CMTMRos is an orange-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation.

What is a MitoTracker?

MitoTracker is a commercially available fluorescent dye (Invitrogen/Molecular Probes) that, like the aforementioned dyes, labels mitochondria within live cells utilizing the mitochondrial membrane potential.

What does MitoTracker deep red measure?

First, MitoTracker Deep Red is actually a mitochondrial potential-dependent dye though this is usually not highlighted in the manufacturer’s manual. It has been used as an index for mitochondrial potential (Lugli et al., 2005; Zhou et al., 2011; Greene et al., 2012).

What are MitoTracker?

MitoTracker is a commercially available fluorescent dye (Invitrogen/Molecular Probes) that, like the aforementioned dyes, labels mitochondria within live cells utilizing the mitochondrial membrane potential. However, MitoTracker is chemically reactive, linking to thiol groups in the mitochondria.