What cell type is used in electroporation?

What cell type is used in electroporation?

It is non-viral, non-toxic and can be used on all cell types including mammalian, bacteria, algae, plant and yeast. It can be used on cells in all forms, in vitro or in vivo/ex vivo. In vitro is Latin for “within glass” and includes suspension cell, tissue slice/whole organ, and adherent cell.

What is the definition of electroporation?

Medical Definition of electroporation : the application of an electric current to a living surface (as the skin or the plasma membrane of a cell) in order to open pores or channels through which something (as a drug or DNA) may pass. Other Words from electroporation.

What are the uses of electroporation method?

Electroporation is also highly efficient for the introduction of foreign genes into tissue culture cells, especially mammalian cells. For example, it is used in the process of producing knockout mice, as well as in tumor treatment, gene therapy, and cell-based therapy.

What is Microprojectile method?

Also known as microprojectile bombardment and particle acceleration. The method used to transform cells using small gold or tungsten particles which are coated with DNA and literally shot into the cell. can be used on either tissue culture cells or seedlings (to make chimeric plants) of any species.

How effective is electroporation?

The electroporation delivery was found to be superior to all other test methods. The maximal enhancement in transfection efficiency by electroporation was up to 30-fold over naked DNA, 5-to 10-fold over liposome-DNA complexes, and over 100-fold over integrin-liposome-DNA complexes.

Is electroporation better than Lipofection?

Interestingly, transformation of all cell lines by electroporation was 7to 10-fold more efficient than lipofectamine dependent on the type of the transformed line. Although after electroporation the cell survival rate was lower, the transformation efficiency was significantly higher.