How many loci are amplified during the Identifiler plus PCR?

How many loci are amplified during the Identifiler plus PCR?

The average stutter ratio and SD values were determined for each of the 15 STR loci amplified by the Identifiler primer set.

What is MiniFiler?

Minifiler is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations including: SWGDAM, DAB, CODIS, ENFSI, Interpol, and GITAD.

What is Identifiler plus?

The Identifiler Plus kit also maintains primer sequences to enable genotyping concordance for samples previously typed using the Identifiler, Identifiler Direct, Profiler Plus, COfiler or SGM Plus kits, and facilitate comparison with existing DNA databases.

How to identify off-ladder alleles?

An allele that sizes only one base pair different from an allele in the allelic ladder will not be incorrectly typed and will be identified as off- ladder.

What alleles are included in the AmpFlSTR™identifiler™allelic ladder?

The AmpFlSTR™Identifiler™Allelic Ladder contains most of the alleles reported for the 15 autosomal loci. Refer to Table 1 on page 12 for a list of the alleles included in the AmpFlSTR™Identifiler™Allelic Ladder.

Can identifiler™kit loci be used to amplify specific alleles?

Since most Identifiler™Kit loci have small size ranges, the potential for preferential amplification of alleles is low. Effect of inhibitorsHeme compounds have been identified as PCR inhibitors in DNA samples extracted from bloodstains (DeFranchis et al.,1988; Akane et al., 1994).

How does the MiniFiler work with identifiler plus?

For heavily degraded samples, the MiniFiler kit has been designed to work in conjunction with the Identifiler Plus kit, utilizing miniSTR technology to help maximize recovery of information.