How do you calculate the accuracy of a pipette?
The most common way to check your pipette accuracy is by weighing water. The density of water is 1 g/mL. This means that every microliter (µL) should weigh 0.001 g. In other words, if your pipette is accurate, the amount of water you dispense will equal the amount the water weighs.
What is the significance of calibrating a Pasteur pipette?
Pipette calibration is necessary to prevent inaccuracies in order to attain better results, performance, and longevity of the pipette itself.
How do you calculate pipette calibration?
Accuracy should be between 99-101%.
- If the pipette is properly calibrated, the calculated value should be very close to the actual value you set on the pipette.
- For example: A = 100 x Vavg/V0 = 100 x 10.019/10 = 100 x 1.0019 = 100.19%
- This particular pipette is properly calibrated.
How is a pipette calibrated?
The calibration of pipette is carried out by gravimetric method. When determining the volume of water, the accuracy of measurements is effected by ambient temperature, atmospheric pressure and relative humidity. These factors are usually combined to give the Z factor, used in calculation of volume of water.
What is the purpose of calibrating a 5 ml volumetric pipette?
Any discrepancy in volumes dispensed may affect the outcomes and reproducibility of an experiment such as qPCR results. It is, therefore, necessary to check pipette calibration every few months to ensure accuracy by dispensing the right volumes.
What is calibrated pipette?
What are the principles of pipette calibration?
Principles of Pipette Calibration. At 20°C temperature and one atmosphere of pressure, water density stays constant at 1 g/mL. The volume of water can be determined by weighing dispensed water. Temperature, atmospheric pressure, and humidity may influence the accuracy of measurements.
How to determine the volume of water in a pipette?
volume of water can be determined by weighting dispensed water. The calibration of pipette is carried out by gravimetric method. When determining the volume of water, the accuracy of measurements is effected by ambient temperature, atmospheric pressure and relative humidity.
How do you measure the accuracy of a pipette?
V = calculated volume of dispensed water. Next, determine the mean value from ten trials. V 0 = theoretical volume you tried to dispense. If the accuracy value lies in the 99-101% range, the pipette is considered normal and calibrated.
How often should I calibrate my pipette?
As a minimum, it’s a good idea to check the calibration of your pipette every 3-6 months. If you are deep-cleaning your pipette, it’s a good idea to check after reassembly too. The more regularly you check your pipette calibration, the sooner you can pick up issues that might affect your results.