Can Bacillus megaterium ferment mannitol?
The medium contains peptic digest of animal tissues and meat extract, which provide nitrogenous compounds. Mannitol serves as the fermentable carbohydrate, fermentation of which can be detected by phenol red. Mannitol fermenting organisms like B. megaterium yield yellow coloured colonies as shown in Figure 4.
What Agar does Bacillus megaterium grow on?
sheep’s blood agar
This photograph depicts the colonial morphology displayed by Gram-positive, Bacillus megaterium bacteria, which was grown on a medium of sheep’s blood agar (SBA), for a 24-hour time period, at a temperature of 37°C.
How do you identify a Bacillus megaterium?
megaterium are concave, smooth and milk white, while B. mycoides looks white, coarser with fine threads around the colony and the color is milk white. The cell morphology (Figure 3 and Table 1) shows that the cell is rod-shaped, gram positive and sporous.
Is Bacillus megaterium Gram-positive or negative?
B. megaterium is a Gram-positive, aerobic spore-forming neutralophilic bacterium found in diverse habitats but commonly regarded as a soil bacterium.
Is Bacillus megaterium facultative anaerobic?
Bacteriology. Members of the genusBacillus are aerobic or facultative anaerobic, gram-positive or gram-variable, spore-forming rods. They are distributed widely in the environment because of the high resistance of their endospores to extreme conditions, including heat, cold, desiccation, salinity, and radiation.
Does Bacillus megaterium produce Acetoin?
It does not produce acetoin nor H2S, and a nitrate/nitrite reduction test was negative. This bacterium produced several extracellular enzymes including protease, cellulase and amylase, but not lipase or esterase under the tested conditions.
What is the morphology of Bacillus megaterium?
Bacillus megaterium is a gram positive, spore producing bacteria. It is a Eubacteria and is found in the soil. It has a rod shape and is one of the largest Eubacteria. Colonies form in chains due to sticky polysaccharides on the cell wall.
How do you identify a Bacillus bacteria?
Diagnosis is confirmed by observation of characteristic encapsulated bacilli in polychrome methylene blue-stained smears of blood, exudate, lymph, cerebrospinal fluid, etc., and/or by culture. Other Bacillus infections are diagnosed by culture of the bacteria.
Is Bacillus megaterium anaerobic?
Bacillus megaterium is a Gram-positive, rod shaped Endospore-forming Bacteria. It is considered Aerobic, but, it is also capable of growing under anaerobic conditions when necessary. One of the largest Eubacteria found in soil, and hence the name “mega” means “relatively big” is a common soil saprophyte.
Is Bacillus megaterium Gram variable?
These gram-positive or gram-variable, aerobic or facultatively anaerobic, rod-shaped bacilli have rounded or squared-off ends, form endospores, tolerate extremes of temperature and moisture, and are ubiquitous. They are found in superficial lake and ocean sediment, even in deep water.
What are bacilli bacteria?
Bacillus species are rod-shaped, endospore-forming aerobic or facultatively anaerobic, Gram-positive bacteria; in some species cultures may turn Gram-negative with age. The many species of the genus exhibit a wide range of physiologic abilities that allow them to live in every natural environment.