How many BP is mCherry?
Structure. The gene for mCherry is 711bp long, and the protein is made up of 236 residues with a mass of 26.722 kDa.
What is mCherry gene?
mCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed. mCherry matures rapidly, making it possible to see results very soon after transfection or activation of transcription. It is highly photostable and resistant to photobleaching (Shaner et al. 2004).
Is mCherry a reporter gene?
Our results identified mCherry as a reliable and quantitative reporter for the study of gene expression in chloroplasts, which is essential for the biotechnological application of microalgal chloroplasts and for improved production of valuable recombinant proteins.
What is a mCherry reporter?
Afp::mCherry is a novel red fluorescent reporter line that provides labeling throughout the VE and its derivatives in the early embryo. The Afp::mCherry line is comparable to our previously reported Afp::GFP line (Kwon et al., 2006; Kwon et al., 2008).
Is mCherry a plasmid?
(Plasmid #20956)
How do you detect mCherry?
It’ll depend on the flow cytometer you have access to but to best detect the mCherry signal excite with the yellow-green laser at 561 nm and detect in the PE-TexasRed channel with a 610/20 bandpass filter.
Is mCherry a dimer?
We found that the brightness values of homo-dimers (normalized to the brightness of the corresponding monomer) for three widely used FPs, namely mEGFP (εdimer = 1.69 ± 0.05), mEYFP (εdimer = 1.63 ± 0.05) and mCherry (εdimer = 1.41 ± 0.04), are generally lower than two, indicating the presence of non-fluorescent …
What laser excites mCherry?
561-nm laser
In addition, the 561-nm laser efficiently excites fruit fluorescent proteins such as mCherry.
Is mCherry the same as tdTomato?
The tandem dimer tdTomato is equally photostable but twice the molecular weight of mCherry, and may be used when fusion tag size does not interfere with protein function.
What is the difference between mCherry and GFP?
The pH-dependent emission profile of GFP and mCherry. GFP is more sensitive to pH change than mCherry. The pH-dependent emission profile of GFP and mCherry was investigated by resuspending GFP and mCherry in phosphate buffers with a pH ranging from 4.4 to 9.1.