What type of DNA is pGLO?
The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably the green fluorescent protein (GFP) and the ampicillin resistance gene.
Is pGLO plasmid DNA?
Download pGLO Sequence (.txt) Bio-Rad’s pGLO plasmid contains DNA sequences that enable its replication and expression of the fluorescent trait (phenotype) in bacteria following transformation. The essential sequences include the following: GFP — jellyfish gene that encodes green fluorescent protein (GFP)
What is the purpose of pGLO?
The pGLO System With the pGLO transformation kit, students use a simple procedure to transform bacteria with a gene that codes for Green Fluorescent Protein (GFP). The real-life source of this gene is the bioluminescent jellyfish Aequorea victoria, and GFP causes the jellyfish to fluoresce and glow in the dark.
Is pGLO a protein?
The pGLO plasmid contains a gene (bla or ampR) for a protein called β-lactamase, which hydrolyzes antibiotics that have a β-lactam ring and makes host cells resistant to compounds like ampicillin. Expression of the β-lactamase gene is constitutive (that is, transcription is always on) from its own promoter site.
What is pGLO biology?
The pGLO plasmid contains an origin or replication, a selectable marker, and the gene for Green Fluorescent Protein (GFP). The plasmid also contains a gene for the arabinose C protein, which is a protein that regulates expression from the arabinose BAD promoter (PBAD).
Who discovered pGLO?
In 1928, a England scientist called Frederick Griffith first discovered this technique, when he working with Streptococcus pneumoniae. Plasmid is a circular DNA molecule, which carrying one or more genes for antibiotic resistance proteins and GFP gene.
How is pGLO made?
In Bio 6B, you’ll work with the plasmid pGLO in a long series of experiments, using multiple techniques of molecular biology. This recombinant plasmid, created by researchers at Bio-Rad, combines a gene for green fluorescent protein (GFP), cloned from a jellyfish, with control elements copied from a bacterial operon.
What is the pGLO plasmid?
Bio-Rad’s pGLO plasmid contains DNA sequences that enable its replication and expression of the fluorescent trait (phenotype) in bacteria following transformation. The essential sequences include the following: GFP — jellyfish gene that encodes green fluorescent protein (GFP)
What is pGLO lab analysis?
pGLO Lab Analysis. Plasmids are pieces of circular DNA that are in bacteria which can code for genes that can cause the bacteria to have extra “features”. With the pGLO plasmid this extra “feature” causes bacteria to glow (from the Green Fluorescent Protein that was inserted) and also has resistance to ampicillin (from the beta-lactamase protein).
What is pGLO transformation in bacteria?
1 Bacterial Transformation with pGlo Overview •Transformation= modification of a bacterium by the uptake and incorporation of exogenous DNA •Determine the transformation efficiency of the competent cells. •Amplify the pGlo expression vector. •Express the pGlo protein.
How to identify the pGLO mutant?
Examine plates and choose on wild-type colony and put colony into the LB broth with ampicillin. Look at plate with the pGLO mutant and look to see if any of the colonies glow green under UV light.
https://www.youtube.com/watch?v=8EBoK1Ft3QA