How do you dilute IAA?

How do you dilute IAA?

IAA Stock preparation: (Example 1mg/ml)

1. Measure 100 mg of IAA in a a test tube or small beaker and add 2-3 ml of distilled ethanol or 1N NaOH to dissolve the powder.
2. Be patient and let it completely dissolve.
3. Very gradually dilute the solution with 10 ml distilled water and make sure solution is homogeneous.

How do you dissolve IAA in water?

To dissolve the IAA completely, add additional 0.25 ml of 1N NaOH. Heating should not be necessary to dissolve the IAA. 2. Once completely dissolved, add the solution dropwise and stir.

How do you make a stock solution of NAA?

To prepare a 1 mg/mL stock solution: Add 100 mg of the plant growth regulator to a 100 mL volumetric flask or other glass container. Add 2-5 mL of solvent to dissolve the powder. Once completely dissolved, bring to volume with double processed water (Product No. W3500).

What is the optimal concentration for IAA elongation?

The optimum concentration was 10−6 M for 4-Cl-IAA and was found not to depend on the time of the growth measurement. However, in the case of IAA the optimum shifted from 10−6 M at the time of maximal growth rate to 10−5 M or even 10−4 M, when growth measured 3–4 hours after auxin application was analysed.

Is IAA soluble in water?

Indole-3-acetic acid

How do you make a ga3 solution?

1. Add 100 mg of Gibberellic Acid 3 Quick-Dissolve™ (GoldBio Catalog # G-120) to a 250 mL beaker. 2. Add 100 ml of molecular biology grade water and stir until dissolved to make a final concentration of 1 mg/ml (1000 ppm).

How do you dissolve 2,4-D?

Generally IAA and 2,4-D are dissolved in a small volume of 95% ethyl alcohol or KOH and then brought to volume with double-distilled water; NAA can be dissolved in a small amount of 1 N NaOH or KOH, which also can be used to dissolve 2,4-D and IAA. The cytokinins are considered to be stable and can be stored at -20°C.

How do you set the pH of a hormone stock solution to 5?

If the hormone used is not heat sensitive, the best way is to add it to the culture medium, then adjust the pH and finally autoclave. If the hormone is heat sensitive, you can test the rate of change and adjust the pH accordingly before autoclaving.

Is NAA soluble in water?

1-Naphthaleneacetic acid (NAA) is an organic compound with the formula C10H7CH2CO2H….1-Naphthaleneacetic acid.

How is IAA standard prepared?

Add 1.0 g of Indole-3-acetic acid (GoldBio Catalog # I-110) to a 100 mL flask or beaker. 2. Add 2.0-5.0 mL of Ethyl Alcohol (EtOH) or 1N NaOH to dissolve the powder. Bring volume to 100 mL with molecular biology grade water.

How does IAA affect root growth?

Different concentrations of IAA appear to have differing effects on root growth. These effects may vary from one species to another but generally at low concentrations IAA stimulates root growth while at higher concentrations it inhibits root growth.

What is the shelf life of auxin stock solutions?

Auxin stock solutions are generally prepared at 100- 1000 times the final desired concentrations. Solution of NAA and 2,4-D can be stored for several months in a refrigerator or indefinitely at -20°C.

What is the role of auxin concentration in plant growth?

The regulation of the auxin concentrations creates a pattern-like system which helps determine plant growth and development. IAA is often used in horticulture to promote adventitious root growth and are used commercially to create root stem cuttings and to promote uniform fruit and flowering growth.

How to prepare indole-3-acetic acid (IAA)?

Preparation of Indole-3-Acetic Acid (IAA) Stock Solution (10 mg/mL) 1. Add 1.0 g of Indole-3-acetic acid (Go ldBio Catalog # I-110) to a 100 mL flask or beaker. 2. Add 2.0-5.0 mL of Ethyl Alcohol (E tOH) or 1N NaOH to dissolve the powder. Bring volume to 100 mL with molecular biology grade water.

What is the function of IAA in plants?

IAA is predominantly produced in the apical bud of and young leaves of plants and is known to be an inducer of cell division and elongation. IAA, as well as most other auxins, are also known to act in concert with, or in opposition to, other plant hormones.