What is the difference between HEK 293 and HEK293T?

What is the difference between HEK 293 and HEK293T?

The key difference between HEK293 and HEK293t is that HEK293 is an immortalized cell line derived from the embryonic human kidney that is transfected with sheared human adenovirus type 5DNA while HEK293t is a daughter cell line derived from HEK293 original cell line that is transfected with a plasmid vector carrying …

What are HEK293F cells?

HEK293F – HEK293F is a variant of HEK293 cells. HEK293F cells were cloned from the HEK293 cell line and adapted to commercial medium. HEK293FT – HEK293 FT is a fast growing variant of HEK293T. HEK293FT cells were cloned from the HEK293T cell line and adapted to commercial media.

Are 293T cells the same as HEK293?

In case of viral production and for other transfections 293T is now preferred since they are normally better transfectable. Hek293T is an inbreed of Hek293 along with Tantigen of SV40, which gave them also added neomycin resistance and Temperature sensitive.

Is HEK293 immortal?

HEK 293 immortal cell line that is comparatively easy to handle. An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division.

Is HEK293 a normal cell line?

Thus, HEK 293T cell line cannot be used as a normal cells (from “HEK293 cell line in cell biology and cancer research: origin, phenotype, karyotype, tumorigenicity”, manuscript in preparation).

How do you get HEK293?

Origins of the HEK293 Cell Line HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.

Where are HEK 293 cells used?

The human embryonic kidney (HEK) 293 cell line and its derivatives are used in experiments ranging from signal transduction and protein interaction studies over viral packaging to rapid small-scale protein expression and biopharmaceutical production.