How much does an Agilent Bioanalyzer cost?

How much does an Agilent Bioanalyzer cost?

Bioanalyzer DNA Analysis

How does the Agilent 2100 Bioanalyzer work?

The Agilent 2100 bioanalyzer automatically calculates the percent ribosomal RNA in mRNA samples and can detect as low as five percent contamination. Ribosomal RNA contamination in mRNA preparations is easily identified by the presence of ribosomal RNA peaks.

What does Rin mean Bioanalyzer?

Bioanalyzer RNA Analysis. RUO. The Bioanalyzer RNA 6000 Nano assay provides reliable and reproducible characterization of total RNA and mRNA from multiple sample types, from as low as 5ng of total RNA. The software will provide an objective measurement of RNA quality with RIN (RNA Integrity Number).

How does a Bioanalyzer quantify RNA?

The Bioanalyzer can be used to look at total RNA quality by observing the 18S and 28S ribosomal peaks and their ratio, or 16S and 23S peaks of prokaryotes. The 28S/18S ratio should be 2, but often is much less with the RNA being of good quality. More important than the ratio is the degradation in the peaks.

How much does a 2100 Bioanalyzer cost?

We have an excellentconditonAgilent 2100 G2938Ccomplete bioanalyzer system from Agilent Technologiesavailable for sale. This model sells new from Agilent for $30,000.

What does a Bioanalyzer measure?

The Bioanalyzer can be used to assess the size and quality of DNA over a range of sizes and concentrations. The DNA 1000 assay can be used with extracted, clean DNA to look at the size profile of a sample from 25bp to 1000bp at a concentration of 0.1-50ng/ul.

What is a good RIN score?

It provides a numerical score (range 1–10) for RNA quality. A higher RIN value indicates a higher degree of RNA integrity [20]. It is generally accepted that for samples with high inter granularity, the RIN level should be about 7–8.

Why is Bioanalyzer used?

Applications. The Bioanalyzer can be used to assess the size and quality of DNA over a range of sizes and concentrations. The DNA 1000 assay can be used with extracted, clean DNA to look at the size profile of a sample from 25bp to 1000bp at a concentration of 0.1-50ng/ul.

How much does Bioanalyzer cost?

This model sells new from Agilent for $30,000. Comes with:Agilent 2100 G2938C Bi… Agilent 2100 Model G2938C Bioanalyzer Complete system-NGS Analysis!

What is the protocol for Bioanalyzer RNA nano chip?

Protocol for Bioanalyzer RNA nano chip. Preparation of material. Prepare a maximum of 12 samples per chip. Maximum concentration recommended is 500ng/µl, 1000ng/µl is okay. Denature RNA 70°C 2min, cool on ice.

What is a Bioanalyzer?

back to protocols The Bioanalyzer is a chip-based capillary electrophoresis machine to analyse RNA, DNA, and protein. It is produced by Agilent and widely used, among other things, in RNA quality control measurements before downstream experiments like microarrays.

How to prepare Agilent RNA 6000 nano assay protocol?

Agilent RNA 6000 Nano 15 Agilent RNA 6000 Nano Assay Protocol 6 Preparing the Gel-Dye Mix. Preparing the Gel-Dye Mix. 1 Allow all reagents to equilibrate to room temperature for 30 minutes before use. Protect the dye concentrate from light while bringing it to room temperature.

How do I clean the electrodes in the Agilent 2100 Bioanalyzer?

1 Slowly fill one of the wells of an electrode cleaner with 350 µl RNaseZAP. 2 Open the lid and place electrode cleaner in the Agilent 2100 bioanalyzer. 3 Close the lid and leave it closed for about 1 minute. 4 Open the lid and remove the electrode cleaner.