What is the selective media for Pseudomonas?
Cetrimide agar is a type of agar used for the selective isolation of the gram-negative bacterium, Pseudomonas aeruginosa.
Does Pseudomonas aeruginosa produce lactose?
P. aeruginosa has few nutritional requirements and can adapt to conditions not tolerated by other organisms. It does not ferment lactose or other carbohydrates but oxidizes glucose and xylose.
What does P. aeruginosa ferment?
P. aeruginosa anaerobically converts pyruvate into lactate, acetate, and succinate. Because pyruvate was found to be essential for anaerobic survival of P. aeruginosa, we analyzed the growth medium for possible pyruvate fermentation products.
Does Pseudomonas aeruginosa grow on MacConkey agar?
Apart from the media mentioned above, Pseudomonas aeruginosa can also be grown in MacConkey agar (a bacterial culture medium commonly used to grow lactose fermenting bacteria). While the bacterium cannot use lactose present in this medium, it survives on peptone.
Why is glycerol added to Cetrimide Agar?
Cetyltrimethylammonium bromide (Cetrimide) is the selective agent and inhibits most bacteria by acting as a detergent. When in contact with bacteria, causes the release of nitrogen and phosphorous from the bacterial cell other than Pseudomonas aeruginosa. Glycerol is supplemented as a source of carbon.
Does Pseudomonas aeruginosa grown on nutrient agar?
It can grow on ordinary media like nutrient agar and grows almost on all the culture media used routinely in the bacteriology lab. Pseudomonas has been seen to grow in distilled water, also. Fig. 28.2: Pseudomonas aeruginosa on nutrient agar showing greenish colouration due to production of Pyoverdin pigment.
Does Pseudomonas putida ferment lactose?
P. putida is a bacterium that is rarely isolated in infections. It exists in soil and is not a part of normal human bacterial flora [10,11]. It is a non-lactose fermenting, oxidase-positive Gram-negative bacilli and a member of the fluorescent group of pseudomonas.
Does sugar feed Pseudomonas?
Mixture of simple sugars inhibits Pseudomonas aeruginosa binding to the apical (AP) surface of polarized epithelium.
Does P. aeruginosa ferment lactose or sucrose?
However, multiple other tests showed that lactose is not fermented by P. aeruginosa….Biochemical characteristics and Enzymes Made.
| Gram Stain | Negative |
|---|---|
| Phenol Red Sucrose | Red: no Sucrose fermentation |
| Phenol Red Lactose | Red: no Lactose fermentation. |
Does P. aeruginosa grow on pea agar?
Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa and Staphylococcus aureus on Phenyl Ethyl Alcohol (PEA) agar, 5 days after innoculation at 37C. PEA agar is a selective medium that permits the growth of gram-positive cocci while inhibiting the growth of most gram-negative organisms.
Does P. aeruginosa grow on blood agar?
In the laboratory, P. aeruginosa can be isolated from any sample (urine, pus, blood, ear swabs, tissue biopsies, body fluids, etc.) They grow well on standard broth and solid media such as blood agar, chocolate agar, and MacConkey agar, which are recommended to isolate Pseudomonas species from clinical specimens.
What is the function of enzymatic digest of gelatin?
• Enzymatic Digest of Gelatin supplies essential nutrients for P. aeruginosa such as nitrogen, vitamins, and carbon. • Magnesium chloride and Potassium chloride stimulates the production of pyocyanin and fluorescein. • Agar is the solidifying agent.
Does Pseudomonas aeruginosa produce pyocyanin and pyorubrin?
Strains of Pseudomonas producing fluorescin but no pyocyanin or pyorubrin were studied by biochemical and antibiotic sensitivity testing. A rapid nitrate test was found to be useful in distinguishing P. aeruginosa (positive) from P. fluorescens and P. putida (both negative). A shortened gelatin test …
What is the biochemical test of Pseudomonas aeruginosa?
Biochemical Test of Pseudomonas aeruginosa Basic Characteristics Properties (Pseudomonas aeruginosa) Capsule Non-Capsulated Catalase Positive (+ve) Cetrimide Test Positive (+ve) Citrate Positive (+ve)
What type of agar is used for Pseudomonas aeruginosa isolation?
Pseudosel Agar or Cetrimide Agar is used for the selective isolation of Pseudomonas aeruginosa from clinical and non-clinical specimens. The medium was first developed by Lowburry and is a modification of Tech Agar.