What is conversion of dUMP to dTMP?
dUMP is converted to dTMP by thymidylate synthase, an enzyme interacting strongly with dCMP deaminase in the multienzyme complex responsible for deoxynucleotide biosynthesis. Human dCMP deaminase, with 178 amino acids and a zinc-binding domain per subunit, is hexameric.
How is thymidine monophosphate formed?
In normal conditions, thymidylate synthetase converts dUMP into thymidine monophosphate using 5,10-methylenetetrahydrofolate (derived from folate) as a methyl group donor. If folate is limiting, there is accumulation of dUMP as the methyl donor, 5,10-methylenetetrahydrofolate is restrictive.
What are the products of thymidylate synthase reaction?
Thymidylate synthase (TS) (EC 2.1. 1.45) is an enzyme that catalyzes the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP).
How is dTMP produced?
The de novo dTMP synthesis pathway catalyzes conversion of deoxyuridylate (dUMP) to dTMP, and involves four enzymes. SHMT1 and MTHFD1 each independently generate 5,10-methylene-tetrahydrofolate from tetrahydrofolate using serine and formate as one-carbon sources, respectively, as described above.
Which of the following is thymidine 5 monophosphate?
DTMP is the neutral species of thymidine 5′-monophosphate (2′-deoxythymidine 5′-monophosphate). It has a role as a fundamental metabolite. It is a conjugate acid of a dTMP(-)….5.1.11H NMR Spectra.
| Spectra ID | 1665 |
|---|---|
| Frequency | 500 MHz |
| Solvent | Water |
| pH | 7.00 |
What is the role of thymidylate synthase?
Thymidylate synthase plays a central role in the biosynthesis of thymidylate, an essential precursor for DNA biosynthesis. In addition to its role in catalysis and cellular metabolism, studies from our laboratory have shown that thymidylate synthase functions as an RNA binding protein.
Where is Deoxyuridine found?
This compound exists in all living organisms and can become part of DNA in both prokaryotic and eukaryotic cells through two mechanisms.