What is the Gram stain of Pasteurella multocida?

What is the Gram stain of Pasteurella multocida?

Pasteurella multocida is a small, gram-negative, nonmotile, non–spore-forming coccobacillus with bipolar staining features. The bacteria typically appear as single bacilli on Gram stain; however, pairs and short chains can also be seen.

Is Pasteurella gram-negative or Gram positive?

Pasteurella are small gram-negative coccobacilli that are primarily commensals or pathogens of animals. However, these organisms can cause a variety of infections in humans, usually as a result of cat scratches, or cat or dog bites or licks.

Is Pasteurella Gram positive?

The members of the genus Pasteurella are small, nonmotile, non-spore-forming, gram-negative organisms. In Gram-stained specimens, they generally appear as a single bacillus, often with bipolar staining, but may also be seen in pairs or short chains (picture 1) [1].

Does Pasteurella multocida grow on MacConkey agar?

They grow best on media containing blood and form small, non-hemolytic, gray colonies. The organism cannot grow on MacConkey agar. Media containing vancomycin, clindamycin, and/or amikacin have been used for selective growth. There are five capsular serogroups and sixteen O antigen serotypes known.

What does Pasteurella bacteria look like?

Pasteurella is a genus of Gram-negative, facultatively anaerobic bacteria. Pasteurella species are nonmotile and pleomorphic, and often exhibit bipolar staining (“safety pin” appearance). Most species are catalase- and oxidase-positive.

How do you identify Pasteurella?

The Pasteurella species are spherical, ovoid or rod-shaped cells 0.3-1.0µm in diameter and 1.0-2.0µm in length. Cells are Gram negative, and occur singly, or in pairs or short chains. Bipolar staining may be seen and capsules may be present. All species are non-motile, and are facultatively anaerobic.

Does Pasteurella multocida grow on blood agar?

Pasteurella is a non-motile aerobe and facultative anaerobe, which grows on chocolate and blood agar, but not on MacConkey agar. Pasteurella multocida does not cause hemolysis on blood agar, and grows in carbon dioxide-rich medium at 37°C [4, 5].

How do you treat Pasteurella multocida in cats?

The acute onset of cellulitis, lymphangitis, and serosanguineous or purulent drainage from hand wounds 12 to 24 hours after cat or dog bites should suggest P. multocida as the predominant etiologic agent. Immediate surgical drainage and penicillin therapy is the treatment of choice.

What is Pasteurella multocida in cats?

Pasteurella species are part of the normal oral flora of cats. They are also a common cause of infection in this species and an important zoonotic agent. INFECTION IN CATS: Pasteurella species are commonly isolated from subcutaneous abscesses and pyothorax in cats.